She S C, Moticka E J
Department of Surgery, Southern Illinois University School of Medicine, Springfield 62794-9230.
Int Ophthalmol. 1993 Feb;17(1):1-7. doi: 10.1007/BF00918860.
Injection of an antigen into the anterior chamber induces an immune response in which antibody production is normal while delayed hypersensitivity reactivity is depressed. Several antigens have been used to induce this response which has been termed anterior chamber associated immune deviation. We have demonstrated that allogeneic lymphocytes injected into the anterior chamber of Lewis rats increase the success rate of subsequent corneal grafts derived from the lymphocyte donor strain. To begin understanding the antigenic requirements of this phenomenon, Wistar/Furth lymphocytes were partially purified into B- and T-cell populations by panning on anti-immunoglobulin coated petri-dishes. These enriched populations were injected separately into the anterior chamber of Lewis rats. Two weeks later these Lewis rats received a full-thickness corneal graft derived from Wistar/Furth donors. Grafts were scored for opacity and neovascularization over the subsequent 8-10 weeks. In control animals injected with balanced salt solution, 20% of the grafts cleared sufficiently to be judged successful. Grafts placed on rats injected with unseparated splenic lymphocytes were judged successful in 75% of the cases. Comparable percentages for grafts on animals injected with B-cell enriched and T-cell enriched populations were 85 and 50 respectively. These results suggest that B cells, which express both class I and class II major histocompatibility antigens are more efficient at inducing anterior chamber associated immune deviation than are T cells, the majority of which express only class I major histocompatibility antigens.
将抗原注入前房可诱导免疫反应，其中抗体产生正常，而迟发型超敏反应性受到抑制。几种抗原已被用于诱导这种反应，该反应被称为前房相关免疫偏离。我们已经证明，将同种异体淋巴细胞注入Lewis大鼠的前房可提高随后来自淋巴细胞供体品系的角膜移植成功率。为了开始了解这种现象的抗原需求，通过在抗免疫球蛋白包被的培养皿上淘选，将Wistar/Furth淋巴细胞部分纯化到B细胞和T细胞群体中。将这些富集的群体分别注入Lewis大鼠的前房。两周后，这些Lewis大鼠接受了来自Wistar/Furth供体的全层角膜移植。在随后的8 - 10周内对移植物的混浊和新生血管形成进行评分。在注射平衡盐溶液的对照动物中，20%的移植物充分清除，被判定为成功。在注射未分离的脾淋巴细胞的大鼠上放置的移植物在75%的病例中被判定为成功。在注射富含B细胞和富含T细胞群体的动物上的移植物的相应百分比分别为8%和50%。这些结果表明，表达I类和II类主要组织相容性抗原的B细胞比大多数仅表达I类主要组织相容性抗原的T细胞在诱导前房相关免疫偏离方面更有效。
