Mizuno K, Clark A F, Streilein J W
Dept Microbiology, University of Miami School of Medicine, FL 33101.
Curr Eye Res. 1988 Jun;7(6):627-32. doi: 10.3109/02713688809031820.
The injection of a variety of antigens, including transplantation antigens, haptens, and viral glycoproteins, into the anterior chamber of eyes of mice produces a characteristic spectrum of immune responses in which suppressed cell mediated immunity dominates. This phenomenon is called Anterior Chamber Associated Immune Deviation (ACAID). Having recently demonstrated that soluble antigens also can induce ACAID in mice, we examined the possibility that an important soluble ocular antigen, retinal S antigen (S Ag) may be capable of inducing ACAID in rats. We have found that injection of S Ag with adjuvant into the anterior chamber of eyes of adult Lewis rats evokes neither a humoral nor a cell mediated immune response that can be detected. However, animals that received S Ag intracamerally in this manner did respond to a subsequent immunogenic dose of S Ag in CFA injected into the hind foot pads, by producing serum anti S Ag antibodies. Importantly, these rats failed to display antigen-specific delayed hypersensitivity. Moreover, lymphoid cells from anterior chamber pre-treated animals, when adoptively transferred, inhibited the development of S Ag-specific delayed hypersensitivity in naive recipients indicating that an active suppression mechanism had been generated. Thus, a soluble antigen injected intracamerally in adult Lewis rats can generate ACAID. The possibility that ACAID may be relevant to the development of S Ag induced uveitis is discussed.
向小鼠眼的前房内注射多种抗原，包括移植抗原、半抗原和病毒糖蛋白，会产生一系列特征性的免疫反应，其中细胞介导的免疫抑制占主导地位。这种现象被称为前房相关免疫偏离（ACAID）。最近我们证实可溶性抗原也能在小鼠中诱导ACAID，于是我们研究了一种重要的可溶性眼部抗原——视网膜S抗原（S Ag）是否能够在大鼠中诱导ACAID。我们发现，将S Ag与佐剂一起注射到成年Lewis大鼠眼的前房内，既不会引发可检测到的体液免疫反应，也不会引发细胞介导的免疫反应。然而，以这种方式在前房内接受S Ag的动物，在随后将免疫原性剂量的S Ag注射到后足垫中的弗氏完全佐剂（CFA）时，会产生血清抗S Ag抗体。重要的是，这些大鼠没有表现出抗原特异性迟发型超敏反应。此外，来自前房预处理动物的淋巴细胞在进行过继转移时，会抑制未接触过抗原的受体中S Ag特异性迟发型超敏反应的发展，这表明已经产生了一种主动抑制机制。因此，向成年Lewis大鼠前房内注射可溶性抗原能够产生ACAID。本文还讨论了ACAID可能与S Ag诱导的葡萄膜炎的发生相关的可能性。
