Jacala A, Adusumalli V E, Kucharczyk N, Sofia R D
Department of Biochemistry, Wallace Laboratories, Division of Carter-wallace, Inc., Cranbury, NJ 08512.
J Chromatogr. 1993 May 5;614(2):285-92. doi: 10.1016/0378-4347(93)80320-4.
An automated, internal standard high-performance liquid chromatographic method for the simultaneous quantitation of felbamate and its three metabolites in adult and neonatal rat brain and heart tissue homogenates was developed and validated. The homogenates prepared from one part of the tissue and four parts of water were extracted with ethyl acetate, and the extract was evaporated to dryness and redissolved in mobile phase. Separation was accomplished on a Waters Resolve C18, 5 microns, 300 mm x 3.9 mm I.D. column with a mobile phase consisting of 0.01 M phosphate buffer, pH 6.8-acetonitrile-methanol (800:150:50, v/v/v). Eluting peaks were monitored with an ultraviolet detector at 210 nm. The linear range of the assay for felbamate and the metabolites was 0.20-50.00 micrograms/ml of homogenate or 1-250 micrograms/g of brain or heart tissue. The lower limit of quantitation for all four analytes was 0.20 micrograms/ml of homogenate or 1.00 micrograms/g of tissue.
建立并验证了一种自动化的、内标高效液相色谱法，用于同时定量测定成年和新生大鼠脑及心脏组织匀浆中的非氨酯及其三种代谢物。将一份组织与四份水制备的匀浆用乙酸乙酯萃取，提取物蒸发至干并重新溶解于流动相中。在 Waters Resolve C18 柱（5 微米，300 毫米×3.9 毫米内径）上进行分离，流动相由 0.01 M 磷酸盐缓冲液（pH 6.8）-乙腈-甲醇（800:150:50，v/v/v）组成。用紫外检测器在 210 纳米处监测洗脱峰。非氨酯及其代谢物的测定线性范围为匀浆 0.20 - 50.00 微克/毫升或脑或心脏组织 1 - 250 微克/克。所有四种分析物的定量下限为匀浆 0.20 微克/毫升或组织 1.00 微克/克。
