Miller T E, Dodd J, Ormrod D J, Geddes R
Department of Medicine, University of Auckland School of Medicine, New Zealand.
Agents Actions. 1993;38 Spec No:C139-42. doi: 10.1007/BF01991164.
Previous laboratory based investigations of a commercially prepared freeze-dried extract of the NZ green-lipped mussel (Perna canaliculus) showed that the material had the capacity to inhibit experimentally induced inflammation. The activity was thought to reside within an aqueous fraction containing high molecular weight material, possibly a polysaccharide. In the present study, a polysaccharide (glycogen) has been extracted from Perna canaliculus and its anti-inflammatory activity examined in an attempt to characterise further the high molecular weight components of this mollusc. Glycogen extracts administered i.v. demonstrated a dose-dependent anti-inflammatory effect in rats with carrageenin-induced footpad oedema. Mobilisation of neutrophils to the site of an inflammatory stimulus was also significantly reduced. This activity was lost if the glycogen extract was treated with KOH or proteinase K, suggesting that the anti-inflammatory properties resided within a protein moiety associated with the glycogen.
此前对市售新西兰绿唇贻贝（Perna canaliculus）冻干提取物进行的基于实验室的研究表明，该物质具有抑制实验性诱导炎症的能力。据认为，活性存在于含有高分子量物质（可能是一种多糖）的水相部分中。在本研究中，已从Perna canaliculus中提取了一种多糖（糖原），并对其抗炎活性进行了检测，以进一步表征这种软体动物的高分子量成分。静脉注射糖原提取物在角叉菜胶诱导的足垫水肿大鼠中显示出剂量依赖性抗炎作用。炎症刺激部位中性粒细胞的动员也显著减少。如果糖原提取物用氢氧化钾或蛋白酶K处理，这种活性就会丧失，这表明抗炎特性存在于与糖原相关的蛋白质部分中。
