Ohta Y, Tanaka M, Terada M, Miller O J, Bank A, Marks P, Rifkind R A
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1232-6. doi: 10.1073/pnas.73.4.1232.
The murine-virus-infected erythroleukemia cell system provides an opportunity to examine regulatory mechanisms controlling cytodifferentiation. A cloned cell line (DR10c3) resistant to the erythropoiesis-inducing effect of dimethylsulfoxide (Me2SO) was isolated from the Me2SO-sensitive line DS19. DR10c3 is characterized as follows: (1) the uptake of [3H]Me2SO is similar to that in DS19; (2) cell growth with and without Me2SO is similar to that of DS19; (3) resistance is relatively stable; (4) the karyotype of DR10c3 reveals an average loss of five chromosomes per cell, but is otherwise similar to that of DS19; (5) total protein and globin synthesis by cells cultured 4 days with or without Me2SO is similar to these syntheses in DS19 cultured without Me2SO; (6) virtually no globin mRNA is detectable after 3 days in Me2SO, as assayed both by RNA-complementary DNA hybridization and by the heterologous cell-free protein-synthesizing system; (7) other polar compounds, N-methylpyrrolidinone, 1-methyl-2-piperidone, N, N-dimethylacetamide, and N-methylacetamide, induce erythroid differentiation in DR10c3, and the accumulation of alpha- and beta-globin chains is indistinguishable from that in DS19; and (8) the concentration optima for induction of differentiation by all these compounds are identical for DR10c3 and DS19.
鼠病毒感染的红白血病细胞系统为研究控制细胞分化的调节机制提供了一个机会。从对二甲基亚砜（Me2SO）敏感的细胞系DS19中分离出了对Me2SO诱导红细胞生成作用具有抗性的克隆细胞系（DR10c3）。DR10c3具有以下特征：（1）[3H]Me2SO的摄取与DS19中的相似；（2）有无Me2SO时的细胞生长与DS19相似；（3）抗性相对稳定；（4）DR10c3的核型显示每个细胞平均丢失五条染色体，但在其他方面与DS19相似；（5）在有或无Me2SO的情况下培养4天的细胞中，总蛋白和珠蛋白的合成与在无Me2SO培养的DS19中的这些合成相似；（6）通过RNA-互补DNA杂交和异源无细胞蛋白质合成系统检测，在Me2SO中培养3天后几乎检测不到珠蛋白mRNA；（7）其他极性化合物，N-甲基吡咯烷酮、1-甲基-2-哌啶酮、N，N-二甲基乙酰胺和N-甲基乙酰胺，可诱导DR10c3中的红细胞分化，α-和β-珠蛋白链的积累与DS19中的无法区分；（8）所有这些化合物诱导分化的最佳浓度对于DR10c3和DS19是相同的。
