Sharpe K L, Vernon M W
Department of Obstetrics and Gynecology, University of Missouri, Columbia 65212.
Biol Reprod. 1993 Jun;48(6):1334-40. doi: 10.1095/biolreprod48.6.1334.
Unique endometriosis-specific secretory proteins would be of paramount importance as noninvasive markers for diagnosis and evaluation of therapeutic approaches for endometriosis. Furthermore, identification of endometriosis-specific secretory proteins may be an important step towards understanding the pathophysiology of endometriosis-associated pain and infertility. Therefore this study was designed to assess protein synthesis and secretion by ectopic uterine implants from steroid-treated and reproductively cyclic rats with surgically induced endometriosis. Uteri, ectopic uterine implants, and control tissues were incubated in L-[35S]methionine or D-[6-3H]glucosamine for 0-24 h and 24-48 h. De novo-synthesized proteins released into the culture media were identified using two-dimensional SDS-PAGE, fluorography, and computer-assisted image analysis. Two distinct groups of ectopic uterine implant proteins were identified: ENDO I (M(r) 40,000-50,000; pI 4.0-5.2) and ENDO II (M(r) 28,000-32,000; pI 7.5-9.0) were produced by ectopic uterine implants and not the uteri. A third group of proteins, previously identified in culture media of the uteri from progesterone-treated rats and called PUP-1 (M(r) 70,000; pI 5.7), was synthesized and secreted by ectopic uterine implants 24-48 h later than in parallel uterine cultures. The detection of ectopic uterine implant proteins suggests biochemical characteristics of the ectopic tissue that may be used to develop unique markers for endometriosis. Furthermore, the delayed synthesis and secretion of the uterine protein PUP-1 by the ectopic uterine implants illustrates yet another example of the asynchronous behavior of these two tissues, which may be related to the etiology or pathophysiology of the disease.
独特的子宫内膜异位症特异性分泌蛋白作为子宫内膜异位症诊断和治疗方法评估的非侵入性标志物至关重要。此外，鉴定子宫内膜异位症特异性分泌蛋白可能是理解子宫内膜异位症相关疼痛和不孕病理生理学的重要一步。因此，本研究旨在评估手术诱导子宫内膜异位症的类固醇处理和生殖周期大鼠的异位子宫植入物的蛋白质合成和分泌情况。将子宫、异位子宫植入物和对照组织在L-[35S]甲硫氨酸或D-[6-3H]葡萄糖胺中孵育0-24小时和24-48小时。使用二维SDS-PAGE、荧光自显影和计算机辅助图像分析鉴定释放到培养基中的新生合成蛋白。鉴定出两组不同的异位子宫植入物蛋白：ENDO I（分子量40,000-50,000；等电点4.0-5.2）和ENDO II（分子量28,000-32,000；等电点7.5-9.0）由异位子宫植入物而非子宫产生。第三组蛋白，先前在孕激素处理大鼠的子宫培养基中鉴定出并称为PUP-1（分子量70,000；等电点5.7），异位子宫植入物合成并分泌该蛋白的时间比平行子宫培养物晚24-48小时。异位子宫植入物蛋白的检测表明异位组织的生化特征，可用于开发子宫内膜异位症的独特标志物。此外，异位子宫植入物对子宫蛋白PUP-1的合成和分泌延迟说明了这两个组织异步行为的又一个例子，这可能与该疾病的病因或病理生理学有关。
