Wilson V L, Taffe B G, Shields P G, Povey A C, Harris C C
Department of Pathology, University of Colorado Health Sciences Center, Denver 80218-1088.
Environ Health Perspect. 1993 Mar;99:261-3. doi: 10.1289/ehp.9399261.
Ionizing radiation produces a variety of damaging insults to nucleic acids, including the promutagenic lesion 8-hydroxydeoxyguanosine. In the present study, the 8-hydroxydeoxyguanosine content of peripheral blood leukocyte DNA isolated from individuals exposed to therapeutic doses of ionizing radiation was determined by a HPLC-coupled 32P-postlabeling assay. Peripheral blood leukocyte DNA from individuals irradiated with 180-200 cGy were observed to contain 2-4.5 times as much 8-hydroxydeoxyguanosine as that from unexposed individuals. These results were confirmed by the use of a HPLC-coupled electrochemical detection system. Thus, human exposure to ionizing radiation significantly increased the circulating leukocyte DNA content of 8-hydroxydeoxyguanosine.
电离辐射会对核酸造成多种损伤，包括诱变前损伤产物8-羟基脱氧鸟苷。在本研究中，通过高效液相色谱联用32P后标记分析法测定了从接受治疗剂量电离辐射的个体中分离出的外周血白细胞DNA的8-羟基脱氧鸟苷含量。观察发现，接受180 - 200 cGy照射的个体外周血白细胞DNA中的8-羟基脱氧鸟苷含量是未受照射个体的2至4.5倍。使用高效液相色谱联用电化学检测系统证实了这些结果。因此，人类暴露于电离辐射会显著增加循环白细胞DNA中8-羟基脱氧鸟苷的含量。
