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ams基因中的ams-1和rne-3071温度敏感突变彼此紧邻，并导致一个类似于大肠杆菌mre基因座产物的结构域内发生替换。

The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

作者信息

McDowall K J, Hernandez R G, Lin-Chao S, Cohen S N

机构信息

Department of Genetics, Stanford University School of Medicine, California 94305.

出版信息

J Bacteriol. 1993 Jul;175(13):4245-9. doi: 10.1128/jb.175.13.4245-4249.1993.

DOI:10.1128/jb.175.13.4245-4249.1993
PMID:8320240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204856/
Abstract

Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cause conservative amino acid substitutions next to a possible nucleotide-binding site within the N-terminal domain of the Ams protein. Computer analysis revealed that the region altered by the mutations has extensive sequence similarity to a predicted gene product from the mre (murein pathway cluster e) locus of E. coli, which has been implicated previously in determining bacterial cell shape.

摘要

在大肠杆菌中，ams（改变的mRNA稳定性）基因中的两个温度敏感突变体ams-1和rne-3071已被广泛用于研究RNA的加工和降解。我们对这些温度敏感等位基因进行了测序，发现这些突变仅相隔6个核苷酸，并在Ams蛋白N端结构域内一个可能的核苷酸结合位点附近引起保守的氨基酸替换。计算机分析表明，被突变改变的区域与大肠杆菌mre（胞壁质途径簇e）位点预测的基因产物具有广泛的序列相似性，该位点先前已被认为与确定细菌细胞形状有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8caf/204856/56aee72bb733/jbacter00055-0340-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8caf/204856/56aee72bb733/jbacter00055-0340-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8caf/204856/56aee72bb733/jbacter00055-0340-a.jpg

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1
The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.ams基因中的ams-1和rne-3071温度敏感突变彼此紧邻，并导致一个类似于大肠杆菌mre基因座产物的结构域内发生替换。
J Bacteriol. 1993 Jul;175(13):4245-9. doi: 10.1128/jb.175.13.4245-4249.1993.
2
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