Korde V M, Phelps T J, Bienkowski P R, White D C
Center for Environmental Biotechnology, University of Tennessee, Knoxville 37932-2567.
Appl Biochem Biotechnol. 1993 Spring;39-40:631-41. doi: 10.1007/BF02919024.
Ground-water contamination by chlorinated aliphatic compounds is a major cause for concern because of their toxicity. This study examined the biodegradation of trichloroethylene and aromatic compounds by microbial consortia enriched from contaminated subsurface sediments. The consortia were capable of utilizing methane and propane as sources of carbon and energy. Two continuously recycled expanded-bed bioreactors were inoculated with (1) the subsurface consortium, and (2) P. fluorescence, P. putida (strains pRB1401 and pWWO), and M. trichosporium OB3b. An uninoculated reactor containing 0.2% sodium azide and 0.5% formalin served as the control. Methane (5% v/v) and propane (3% v/v) were maintained by batch feeding through the course of the experiment. Greater than 97% degradation of trichloroethylene was observed over a period of 12 d. More than 99% of benzene, toluene, and xylene were degraded within the first 7 d. Dissolved oxygen levels were measured and found to be in the range 4.9-6.5 mg/L throughout the experiments.
由于其毒性，氯化脂肪族化合物对地下水的污染是一个主要的关注问题。本研究考察了从受污染的地下沉积物中富集的微生物群落对三氯乙烯和芳香族化合物的生物降解作用。这些群落能够利用甲烷和丙烷作为碳源和能源。两个连续循环膨胀床生物反应器分别接种了（1）地下群落，以及（2）荧光假单胞菌、恶臭假单胞菌（菌株pRB1401和pWWO）和 trichosporium OB3b 菌。一个含有0.2%叠氮化钠和0.5%福尔马林的未接种反应器用作对照。在实验过程中，通过分批进料维持甲烷（5% v/v）和丙烷（3% v/v）。在12天的时间内，观察到三氯乙烯的降解率大于97%。在最初的7天内，超过99%的苯、甲苯和二甲苯被降解。在整个实验过程中，测量了溶解氧水平，发现其范围在4.9 - 6.5 mg/L之间。
