Malinski T, Taha Z, Grunfeld S, Patton S, Kapturczak M, Tomboulian P
Department of Chemistry, Oakland University, MI 48309-4401.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1076-82. doi: 10.1006/bbrc.1993.1735.
Porphyrinic sensors were used for the in situ monitoring of nitric oxide release and diffusion in the endothelial cell, as well as its subsequent diffusion from the endothelial cell through the muscle cells found in the rabbit aorta. The experimental data was compared with that predicted based on Fick's equation for linear diffusion. A time delay of 1.5 s between prediction and experimental concentration of NO due to its chemical reactions was observed at the distance of 100 microns from endothelial cell. About 37% of the NO produced is consumed in chemical reactions in the aorta.
卟啉传感器用于原位监测一氧化氮在内皮细胞中的释放和扩散，以及其随后从内皮细胞通过兔主动脉中的肌肉细胞的扩散。将实验数据与基于菲克线性扩散方程预测的数据进行了比较。在距内皮细胞100微米处，由于化学反应，一氧化氮预测浓度与实验浓度之间观察到1.5秒的时间延迟。所产生的一氧化氮约37%在主动脉的化学反应中被消耗。
