Statland B E, Winkel P, Killingsworth L M
Clin Chem. 1976 Oct;22(10):1635-8.
Using an automated immunoprecipitin method, we assayed human sera for 10 proteins: haptoglobin, orosomucoid, transferrin, alpha1 antitrypsin, alpha2-macroglobulin, IgG, IGa, IgM, complement C3, and complement C4. Blood from 14 healthy subjects (25-40y) was sampled on six separate days. From each venipuncture serum was divided into four eliquots; two were assayed on the day of venipuncture and two were frozen and kept until the end of the study, when all of the frozen samples were analyzed in one batch. With this experimental design, batch-to-batch analytical variation could be estimated, and we avoided confounding it with the biological variation. Data analysis was based on the analysis of variance technique. The average physiological intra-individual coefficient of variation ranged from 2.5% for transferrin to 11.1% for orosomucoid. THe interindividual variation ranged from 9.5% for transferrin to 70.5% for haptoglobin and the ratio between intra-individual variation and interindividual variation ranged from 0.66 for IgM to 0.26 for orosomucoid and transferrin.
我们采用自动免疫沉淀法对人血清中的10种蛋白质进行了检测：触珠蛋白、类粘蛋白、转铁蛋白、α1抗胰蛋白酶、α2巨球蛋白、免疫球蛋白G、免疫球蛋白A、免疫球蛋白M、补体C3和补体C4。从14名健康受试者（25 - 40岁）采集血液，分6天进行采样。每次静脉穿刺采集的血清均分为4份；两份在静脉穿刺当天进行检测，两份冷冻保存直至研究结束，届时所有冷冻样本集中进行分析。通过这种实验设计，可以估算批次间的分析变异，且我们避免了将其与生物学变异混淆。数据分析基于方差分析技术。个体内平均生理变异系数范围为：转铁蛋白2.5%至类粘蛋白11.1%。个体间变异范围为：转铁蛋白9.5%至触珠蛋白70.5%，个体内变异与个体间变异的比值范围为：免疫球蛋白M 0.66至类粘蛋白和转铁蛋白0.26。
