试剂条指示剂与尿蛋白之间的结合特性。

Characteristics of binding between reagent-strip indicators and urinary proteins.

作者信息

Bowie L, Smith S, Gochman N

出版信息

Clin Chem. 1977 Jan;23(1):128-30.

Abstract

We describe the binding characteristics of two indicator dyes that are widely used in commercial "dip-stick" indicators for protein in urine. Binding was studied by both gel filtration chromatography and by spectrophotometric titrations. The results demonstrate that the well-known lack of sensitivity of these strips to proteins other than albumin relates to the number and affinity of binding sites on these proteins as compared to albumin. The data suggest that these test strips can screen more effectively for albuminuria than for other proteinurias.

摘要

我们描述了两种指示剂染料的结合特性，它们广泛用于尿液蛋白质的商业“试纸条”指示剂中。通过凝胶过滤色谱法和分光光度滴定法研究了结合情况。结果表明，这些试纸条对白蛋白以外的蛋白质缺乏敏感性，这与这些蛋白质与白蛋白相比的结合位点数量和亲和力有关。数据表明，这些试纸条对蛋白尿的筛查比对其他蛋白尿更有效。