Sasaki M, Pugia M J, Parker D R, Kuromoto K, Furukawa I, Konishi I
Clinical Laboratory, Minoh City Hospital, Minoh City, Osaka, Japan.
J Clin Lab Anal. 1999;13(5):246-50. doi: 10.1002/(SICI)1098-2825(1999)13:5<246::AID-JCLA10>3.0.CO;2-W.
An albumin selective urine strip based on bis (3',3''-diiodo-4', 4''-dihydroxy-5',5''-dinitrophenyl)-3,4,5,6-tetrabromo sulfonphthalein dye (DIDNTB) dye was examined in populations with clinical proteinuria. The relationship of albumin to the sum concentration of all protein in urine was found to vary widely even though the albumin concentration generally increased with the total protein concentration. The albumin reagent strips correlated well with immuno-nephrometric assays for albumin on specimens from hypertensives, diabetics, and renal disease which tended to have albumin contents of >/= 50.0%. High proteinuria concentrations of > 250 mg/l, with low albumin contents of </= 30%, occurred more frequently in cases of cancer, infection, and myeloma. The albumin strip read higher than the immuno assay in samples with high proteinuria and low albuminuria. The albumin strip was also less affected by albumin fragmentation than by the immunological assay. Overall, the albumin strip gave a lower risk of false negatives than a protein strip based on tetrabromophenol blue (TBPB) dye and was more sensitive to disease condition. The protein strip was not sensitive to low levels of albumin and the agreement between TBPB dye strip and the quantitative analysis was not as affected by the albumin content.
基于双（3'，3'' - 二碘 - 4'，4'' - 二羟基 - 5'，5'' - 二硝基苯基） - 3,4,5,6 - 四溴磺酞染料（DIDNTB）的白蛋白选择性尿试纸条在临床蛋白尿人群中进行了检测。尽管白蛋白浓度通常随总蛋白浓度增加，但发现尿中白蛋白与所有蛋白质总浓度的关系差异很大。白蛋白试剂条与免疫比浊法检测高血压、糖尿病和肾病患者标本中的白蛋白相关性良好，这些患者的白蛋白含量往往≥50.0%。蛋白尿浓度>250mg/l且白蛋白含量≤30%的情况在癌症、感染和骨髓瘤患者中更常见。在高蛋白尿和低白蛋白尿的样本中，白蛋白试纸条的读数高于免疫测定法。与免疫测定法相比，白蛋白试纸条受白蛋白片段化的影响也较小。总体而言，与基于四溴酚蓝（TBPB）染料的蛋白试纸条相比，白蛋白试纸条出现假阴性的风险更低，对疾病状况更敏感。蛋白试纸条对低水平白蛋白不敏感，TBPB染料试纸条与定量分析之间的一致性受白蛋白含量的影响较小。
