Demacker P N, Dormans T P, Koenders E B, Corstens F H
Department of General Internal Medicine, University Hospital Nijmegen, The Netherlands.
J Nucl Med. 1993 Aug;34(8):1316-21.
Scintigraphic detection of atherosclerotic lesions using 111In-polyclonal IgG was studied. In Watanabe heritable hyperlipidemic (WHHL) rabbits, an animal model for hypercholesterolemia with spontaneous atherosclerosis, aged WHHL rabbits incorporated more 111In-IgG into atherosclerotic lesions than young WHHL or control NZW rabbits. This result is in agreement with histological analysis. However, due to the low ratio of lesion-incorporated radioactivity to circulating radioactivity, in vivo gamma imaging of atherosclerosis with 111In-IgG scintigraphy was unsuccessful. Interventional agents, Probucol or vitamin E, used for 28 days to reduce the amount of autoantibodies produced against biological modified low-density lipoproteins did not produce differences in 111In-IgG incorporation into the aorta ex vivo. Ethinylestradiol, used for 28 days, exhibited similar incorporation with decreased serum cholesterol by 45%. Although atherosclerosis histology and lesion surfaces of WHHL rabbits are similar to those in adult humans, it is obvious that noninvasive gamma imaging with polyclonal 111In scintigraphy is not reliable for serial evaluation of the extent of atherosclerosis. Our results emphasize the need to develop pharmaceuticals to image atherosclerosis.
研究了使用¹¹¹铟标记的多克隆免疫球蛋白G（¹¹¹In - 多克隆IgG）进行闪烁扫描检测动脉粥样硬化病变。在渡边遗传性高脂血症（WHHL）兔中，这是一种具有自发性动脉粥样硬化的高胆固醇血症动物模型，老年WHHL兔比年轻的WHHL兔或对照新西兰白兔（NZW兔）在动脉粥样硬化病变中摄取更多的¹¹¹In - IgG。这一结果与组织学分析一致。然而，由于病变摄取放射性与循环放射性的比例较低，¹¹¹In - IgG闪烁扫描对动脉粥样硬化进行体内γ成像未成功。使用普罗布考或维生素E等干预剂28天以减少针对生物修饰的低密度脂蛋白产生的自身抗体量，离体情况下对¹¹¹In - IgG在主动脉中的摄取没有产生差异。使用28天的炔雌醇表现出类似的摄取情况，同时血清胆固醇降低了45%。尽管WHHL兔的动脉粥样硬化组织学和病变表面与成年人类相似，但很明显，¹¹¹In多克隆闪烁扫描的无创γ成像对于连续评估动脉粥样硬化程度并不可靠。我们的结果强调了开发用于动脉粥样硬化成像的药物的必要性。
