Van Aelst L, Barr M, Marcus S, Polverino A, Wigler M
Cold Spring Harbor Laboratory, NY 11724.
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6213-7. doi: 10.1073/pnas.90.13.6213.
We used a Saccharomyces cerevisiae genetic system to detect the physical interaction of RAS and RAF oncoproteins. We also observed interaction between RAS and byr2, a protein kinase implicated as a mediator of the Schizosaccharomyces pombe ras1 protein. Interaction with RAS required only the N-terminal domains of RAF or byr2 and was disrupted by mutations in either the guanine nucleotide-binding or effector-loop domains of RAS. We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed.
我们使用酿酒酵母遗传系统来检测RAS和RAF癌蛋白之间的物理相互作用。我们还观察到RAS与byr2之间的相互作用，byr2是一种蛋白激酶，被认为是粟酒裂殖酵母ras1蛋白的介质。与RAS的相互作用仅需要RAF或byr2的N末端结构域，并且被RAS的鸟嘌呤核苷酸结合或效应环结构域中的突变破坏。我们观察到MEK（一种磷酸化丝裂原活化蛋白激酶的激酶）与RAF的催化结构域之间的相互作用。RAS和MEK也相互作用，但仅在RAF过表达时才会发生。
