Meugnier H, Fernandez M P, Bes M, Brun Y, Bornstein N, Freney J, Fleurette J
Centre National de Référence des Staphylocoques, Faculté de Médecine Alexis Carrel, Lyon, France.
Res Microbiol. 1993 Jan;144(1):25-33. doi: 10.1016/0923-2508(93)90212-k.
rRNA gene restriction patterns (ribotyping) were compared with phage typing, serotyping, enterotoxins and exfoliatin production in the analysis of 26 Staphylococcus aureus strains isolated from two different nosocomial outbreaks. Total DNA was cleaved by EcoRI restriction endonuclease. After agarose gel electrophoresis and Southern transfer, the hybridization of the membranes was done with radiolabelled 16S rRNA gene from Bacillus subtilis inserted into a plasmid vector. Six to 13 fragments were visualized. A core of common fragments was discerned for all strains tested. A full correlation between ribotyping and conventional markers was observed in only one of the outbreaks studied. In both outbreaks, ribotyping proved helpful in characterizing otherwise untypable strains.
在对从两次不同医院感染暴发中分离出的26株金黄色葡萄球菌进行分析时，将核糖体RNA（rRNA）基因限制性图谱（核糖分型）与噬菌体分型、血清分型、肠毒素和剥脱毒素产生情况进行了比较。总DNA用EcoRI限制性内切酶切割。琼脂糖凝胶电泳和Southern转移后，用插入质粒载体的来自枯草芽孢杆菌的放射性标记16S rRNA基因对膜进行杂交。观察到6至13条片段。在所测试的所有菌株中都识别出了一个共同片段核心。在所研究的两次暴发中，仅在其中一次中观察到核糖分型与传统标记之间的完全相关性。在两次暴发中，核糖分型都被证明有助于鉴定其他方法无法分型的菌株。
