Pekkala D H, Low D E, Wyper P A, Rose D, Sturman D, Pritchard M F, Panaro L, Simor A E
Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada.
Diagn Microbiol Infect Dis. 1992 May-Jun;15(4):307-11. doi: 10.1016/0732-8893(92)90016-m.
Outbreaks of Staphylococcus aureus infections in neonatal units require prompt investigation and implementation of control measures. From January to March 1990, a marked increase in the number of S. aureus infections was observed in a neonatal nursery. Twenty-seven S. aureus isolates from 23 patients were analyzed by phage typing and restriction endonuclease analysis (REA). Only nine strains were differentiated by phage type. However, REA with HindIII, CfoI, and ClaI differentiated 20 strains. The REA results indicated that the outbreak was due to several different S. aureus strains and did not represent transmission of a single epidemic strain. REA may enable more accurate determination of the presence or absence of an epidemic strain during an outbreak than would traditional methods such as phage typing.
新生儿病房中金黄色葡萄球菌感染的暴发需要迅速进行调查并实施控制措施。1990年1月至3月，在一家新生儿保育室中观察到金黄色葡萄球菌感染的数量显著增加。对来自23名患者的27株金黄色葡萄球菌分离株进行了噬菌体分型和限制性内切酶分析（REA）。通过噬菌体分型仅区分出9株菌株。然而，用HindIII、CfoI和ClaI进行的REA区分出了20株菌株。REA结果表明，此次暴发是由几种不同的金黄色葡萄球菌菌株引起的，并非单一流行菌株的传播。与噬菌体分型等传统方法相比，REA在暴发期间可能能够更准确地确定流行菌株的存在与否。
