Yerle M, Lahbib-Mansais Y, Thomsen P D, Gellin J
INRA, Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France.
Anim Genet. 1993 Apr;24(2):129-31. doi: 10.1111/j.1365-2052.1993.tb00254.x.
The gene encoding the porcine growth hormone (GH) has been localized to the q-arm of chromosome 12 using high-resolution R-banded chromosomes for in situ hybridization. We report here the localization of GH on the p-arm of this chromosome when using in situ hybridization on high-resolution G-banded chromosomes. Sequential Q- and R-banding show that this discrepancy is caused by a reversed orientation of chromosome 12 in the R-banded high-resolution karyotype published by Rønne et al. (1987) and the G-banded standard karyotype.
利用高分辨率R带染色体进行原位杂交,已将编码猪生长激素(GH)的基因定位到12号染色体的q臂上。在此我们报告,当使用高分辨率G带染色体进行原位杂交时,GH定位在该染色体的p臂上。连续的Q带和R带显示,这种差异是由Rønne等人(1987年)发表的R带高分辨率核型和G带标准核型中12号染色体的反向排列造成的。