Lahbib-Mansais Y, Yerle M, Pinton P, Gellin J
Laboratoire de Genetique Cellulaire, Centre INRA Toulouse, France.
Mamm Genome. 1996 Mar;7(3):174-9. doi: 10.1007/s003359900049.
Four homeobox genes that belong to the four homeobox gene clusters known in mammals have been regionally assigned to four distinct porcine chromosomes in conserved regions between human and pig. HOXA11, HOXB6, HOXC8, and HOXD4 genes were mapped by radioactive in situ hybridization to porcine Chromosomes (Chrs) 18q21-24 (with a secondary signal in 16q14-21), 12p11-12, 5p11-12, and 15q22-23 respectively. Besides, we have also revealed the presence of a porcine homeobox (pig Hbx24) which, although showing DNA sequence homology with a mouse gene of HOXB cluster, was located on porcine Chr 3 (3p14-13) outside the Hox clusters. To support the identity of the homeobox gene clusters analyzed and in the light of the high sequence similarity among homeobox genes, we also localized markers known to be mapped near each Hox cluster in human. In this way, four genes were also mapped in pig: GAPD (5q12-21), GAD1 (15q21-22), INHBA (18q24), and IGFBP3 (18q24). Mapping of HOXA11, INHBA, and IGFBP3 on pig Chr 18 constitutes the first assignments of genes on this small chromosome. These new localizations extend the information on the conservation of four human chromosomal regions in the pig genome.
属于哺乳动物中已知的四个同源框基因簇的四个同源框基因已被区域定位到人与猪之间保守区域内的四条不同的猪染色体上。通过放射性原位杂交将HOXA11、HOXB6、HOXC8和HOXD4基因分别定位到猪的18号染色体q21 - 24区域（在16号染色体q14 - 21区域有一个次要信号）、12号染色体p11 - 12区域、5号染色体p11 - 12区域和15号染色体q22 - 23区域。此外，我们还发现了一个猪同源框基因（猪Hbx24），尽管它与HOXB簇的一个小鼠基因具有DNA序列同源性，但它位于猪3号染色体（3p14 - 13）上，在Hox簇之外。为了支持所分析的同源框基因簇的一致性，并鉴于同源框基因之间的高度序列相似性，我们还定位了已知在人类中位于每个Hox簇附近的标记。通过这种方式，四个基因也在猪中进行了定位：GAPD（5q12 - 21）、GAD1（15q21 - 22）、INHBA（18q24）和IGFBP3（18q24）。HOXA11、INHBA和IGFBP3在猪18号染色体上的定位是该小染色体上基因的首次定位。这些新的定位扩展了关于猪基因组中四个人类染色体区域保守性的信息。
