登革病毒诱导的抑制性细胞因子在巨噬细胞和淋巴细胞上的特异性受体。
Specific receptors for dengue virus-induced suppressor cytokine on macrophages and lymphocytes.
作者信息
Mukherjee R, Chaturvedi P, Chaturvedi U C
机构信息
Postgraduate Department of Microbiology, K. G. Medical College, Lucknow, India.
出版信息
Int J Exp Pathol. 1994 Feb;75(1):29-36.
The present study was undertaken to investigate the presence of receptors for the dengue virus-induced suppressor cytokine (SF) on mouse macrophages (Mphi), T and B lymphocytes. It was observed that 125I-SF bound to T and B cells to a variable degree through both high and low affinity receptors. The numbers of high affinity receptors on T and B cells were 35,000 and 16,000/cell respectively and those of low affinity receptors were 0.72 x 10(6) and 0.33 x 10(6)/cell respectively. The binding of 125I-SF was also shown by removal of the biological activity following adsorption on Mphi monolayers. The suppressor activity was inhibited by pretreatment of SF with anti-SF-antisera. The binding of 125I-SF to Mphi was saturable, reversible and dependent on pH, time and temperature. This shows the presence of a specific receptor for SF on mouse Mphi, T and B lymphocytes.
本研究旨在调查登革病毒诱导的抑制性细胞因子（SF）在小鼠巨噬细胞（Mphi）、T淋巴细胞和B淋巴细胞上的受体存在情况。观察到125I-SF通过高亲和力和低亲和力受体与T细胞和B细胞发生不同程度的结合。T细胞和B细胞上高亲和力受体的数量分别为35,000个/细胞和16,000个/细胞，低亲和力受体的数量分别为0.72×10⁶个/细胞和0.33×10⁶个/细胞。125I-SF吸附于Mphi单层细胞后生物活性消失，也表明了其结合情况。用抗SF抗血清预处理SF可抑制其抑制活性。125I-SF与Mphi的结合具有饱和性、可逆性，且依赖于pH、时间和温度。这表明小鼠Mphi、T淋巴细胞和B淋巴细胞上存在SF的特异性受体。
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