Schulz W A, Ebling B, Hasse A, Zenke F, Breunig K
Institut für Physiologische Chemie I, Heinrich-Heine-Universität, Düsseldorf.
Biol Chem Hoppe Seyler. 1993 May;374(5):313-8. doi: 10.1515/bchm3.1993.374.1-6.313.
Expression of the LAC9 gene from the yeast Kluyveromyces lactis in HepG2 human hepatoblastoma cells efficiently induced luciferase expression from reporter plasmids containing the four LAC9 binding sites from the K. lactis GAL1-GAL10 gene linked to a basal promoter. Induction was approximately 100fold and was dependent on the presence of the UAS sequence and an intact reading frame in the LAC9 gene. Additional cotransfection of constructs expressing the K. lactis GAL80 gene reduced luciferase activity by up to 98%. This inhibition was not affected by addition of 14mM galactose to the medium. No further yeast-specific factors appear necessary for efficient inhibition of LAC9 by GAL80, but additional gene products may be required for activation by galactose.
乳酸克鲁维酵母的LAC9基因在人肝癌细胞HepG2中的表达，能有效地诱导含有来自乳酸克鲁维酵母GAL1 - GAL10基因的四个LAC9结合位点并与基础启动子相连的报告质粒的荧光素酶表达。诱导倍数约为100倍，且依赖于UAS序列的存在和LAC9基因中完整的阅读框。额外共转染表达乳酸克鲁维酵母GAL80基因的构建体可使荧光素酶活性降低高达98%。这种抑制不受向培养基中添加14mM半乳糖的影响。似乎不需要其他酵母特异性因子来实现GAL80对LAC9的有效抑制，但半乳糖激活可能需要其他基因产物。
