Luskin M B
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322.
Neuron. 1993 Jul;11(1):173-89. doi: 10.1016/0896-6273(93)90281-u.
The subventricular zone of the postnatal forebrain produces mainly glia, although it supports limited neurogenesis. To determine whether the subventricular zone is positionally specified, the phenotype and destination of the progeny of subventricular zone cells along the anterior-posterior axis of the lateral ventricles were analyzed. A retroviral lineage tracer containing the E. coli reporter gene lacZ was injected into different parts of the subventricular zone of neonatal rat pups, and at various times thereafter, the expression of beta-galactosidase was detected histochemically or immunohistochemically in the descendants of infected cells. A discrete region of the anterior part of the subventricular zone (SVZa) generated an immense number of neurons that differentiated into granule cells and periglomerular cells of the olfactory bulb-the two major types of interneurons. Thus, the SVZa appears to constitute a specialized source of neuronal progenitor cells. To reach the olfactory bulb, neurons arising in the SVZa migrate several millimeters along a highly restricted route. Guidance cues must be involved to prohibit widespread dispersion of these migrating neurons.
出生后前脑的脑室下区主要产生神经胶质细胞，尽管它也支持有限的神经发生。为了确定脑室下区是否在位置上具有特异性，分析了脑室下区细胞后代沿侧脑室前后轴的表型和去向。将携带大肠杆菌报告基因lacZ的逆转录病毒谱系示踪剂注射到新生大鼠幼崽脑室下区的不同部位，此后在不同时间，通过组织化学或免疫组织化学方法在感染细胞的后代中检测β-半乳糖苷酶的表达。脑室下区前部（SVZa）的一个离散区域产生了大量神经元，这些神经元分化为嗅球的颗粒细胞和球周细胞——两种主要的中间神经元类型。因此，SVZa似乎构成了神经元祖细胞的一个特殊来源。为了到达嗅球，起源于SVZa的神经元沿着高度受限的路径迁移数毫米。必须有引导线索来阻止这些迁移神经元的广泛扩散。
