• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过单体和蛋白A纯化的多克隆人免疫球蛋白改善葡萄球菌感染的检测

Improved detection of a staphylococcal infection by monomeric and protein A-purified polyclonal human immunoglobulin.

作者信息

Calame W, Welling M, Feitsma H I, Ensing G J, Pauwels E K

机构信息

Department of Diagnostic Radiology and Nuclear Medicine, University Hospital, Leiden, The Netherlands.

出版信息

Eur J Nucl Med. 1993 Jun;20(6):490-4. doi: 10.1007/BF00175161.

DOI:10.1007/BF00175161
PMID:8339732
Abstract

The present study was undertaken to compare the technetium-99m labelled non-specific polyclonal human immunoglobulin (Ig) with 99mTc-labelled monomeric human immunoglobulin (m-Ig), 99mTc-labelled, protein A-purified, human immunoglobulin (A-Ig) and 99mTc-labelled monomeric, protein A-purified, human immunoglobulin (mA-Ig) as tracer agents for the detection of a thigh infection with Staphylococcus aureus. In vitro the binding of the various tracer agents to bacteria at various intervals was determined. For the in vivo evaluation, mice were infected and received one of the various labelled proteins. Scintigrams were made 0.25, 1, 4 and 24 h later. All 99mTc-labelled Igs bound to bacteria in vitro: the percentages of binding for the m-Ig (from 1 h onwards) and A-Ig and mA-Ig (from 3 h onwards) were significantly higher than that for Ig. The in vivo target-to-non-target (T/NT) ratios were significantly higher from 4 h onwards for all purified Igs than for Ig. Protein A-purified Igs yielded higher T/NT ratios than m-Ig. Furthermore, the amount of activity in the liver was significantly lower 24 h after administration of m-Ig, A-Ig and mA-Ig than after administration of Ig. It is concluded that in this experimental infection 99mTc-labelled monomeric Ig localizes a staphylococcal thigh infection better and faster than 99mTc-labelled unpurified Ig. However, the accumulation obtained with protein A-purified Ig or protein A-purified monomeric Ig was the highest of all tracer agents tested.

摘要

本研究旨在比较锝-99m标记的非特异性多克隆人免疫球蛋白（Ig）与锝-99m标记的单体人免疫球蛋白（m-Ig）、锝-99m标记的蛋白A纯化的人免疫球蛋白（A-Ig）以及锝-99m标记的单体、蛋白A纯化的人免疫球蛋白（mA-Ig）作为检测金黄色葡萄球菌引起的大腿感染的示踪剂。在体外，测定了不同示踪剂在不同时间间隔与细菌的结合情况。为了进行体内评估，将小鼠感染并给予各种标记蛋白之一。在0.25、1、4和24小时后进行闪烁扫描。所有锝-99m标记的Ig在体外均能与细菌结合：m-Ig（从1小时起）、A-Ig和mA-Ig（从3小时起）的结合百分比显著高于Ig。从4小时起，所有纯化Ig的体内靶标与非靶标（T/NT）比率均显著高于Ig。蛋白A纯化的Ig产生的T/NT比率高于m-Ig。此外，给予m-Ig、A-Ig和mA-Ig 24小时后肝脏中的活性量显著低于给予Ig后。得出的结论是，在这种实验性感染中，锝-99m标记的单体Ig比锝-99m标记的未纯化Ig能更好、更快地定位金黄色葡萄球菌引起的大腿感染。然而，在所有测试的示踪剂中，蛋白A纯化的Ig或蛋白A纯化的单体Ig的积聚量最高。

相似文献

1
Improved detection of a staphylococcal infection by monomeric and protein A-purified polyclonal human immunoglobulin.通过单体和蛋白A纯化的多克隆人免疫球蛋白改善葡萄球菌感染的检测
Eur J Nucl Med. 1993 Jun;20(6):490-4. doi: 10.1007/BF00175161.
2
Optimized localization of bacterial infections with technetium-99m labelled human immunoglobulin after protein charge selection.蛋白质电荷选择后，用锝-99m标记的人免疫球蛋白对细菌感染进行优化定位。
Eur J Nucl Med. 1994 Oct;21(10):1135-40. doi: 10.1007/BF00181070.
3
Detection of a local staphylococcal infection in mice with technetium-99m-labeled polyclonal human immunoglobulin.用99m锝标记的多克隆人免疫球蛋白检测小鼠局部葡萄球菌感染。
J Nucl Med. 1991 Mar;32(3):468-74.
4
Technetium-99m labelled hydrazinonicotinamido human non-specific polyclonal immunoglobulin G for detection of infectious foci: a comparison with two other technetium-labelled immunoglobulin preparations.锝-99m标记的肼基烟酰胺人非特异性多克隆免疫球蛋白G用于检测感染灶：与其他两种锝标记免疫球蛋白制剂的比较
Eur J Nucl Med. 1996 Apr;23(4):414-21. doi: 10.1007/BF01247370.
5
Binding of 99mTc-labelled polyclonal human immunoglobulin to bacteria as a mechanism for scintigraphic detection of infection.99mTc标记的多克隆人免疫球蛋白与细菌的结合作为感染闪烁显像检测的一种机制。
Eur J Nucl Med. 1991;18(6):396-400. doi: 10.1007/BF02258430.
6
Technetium-99m labelled antimicrobial peptides discriminate between bacterial infections and sterile inflammations.锝-99m标记的抗菌肽可区分细菌感染和无菌性炎症。
Eur J Nucl Med. 2000 Mar;27(3):292-301. doi: 10.1007/s002590050036.
7
Imaging of bacterial infections with 99mTc-labeled human neutrophil peptide-1.用99mTc标记的人中性粒细胞肽-1对细菌感染进行成像。
J Nucl Med. 1999 Dec;40(12):2073-80.
8
Retention of technetium-99m in infectious foci in rats after release from technetium-99m labelled human non-specific polyclonal immunoglobulin G: a dual-label study with hydrazinonicotinamido and iminothiolano immunoglobulin.从锝-99m标记的人非特异性多克隆免疫球蛋白G释放后大鼠感染灶中锝-99m的滞留：用肼基烟酰胺和亚氨基硫代乳酸免疫球蛋白进行的双标记研究
Eur J Nucl Med. 1996 Nov;23(11):1536-9. doi: 10.1007/BF01254481.
9
Localization of a bacterial infection with 99Tcm-labelled human IgG: further improvement with enriched IgG subclass preparations.用99锝标记的人免疫球蛋白定位细菌感染：用富集免疫球蛋白亚类制剂进一步改善
Nucl Med Commun. 1997 Nov;18(11):1057-64. doi: 10.1097/00006231-199711000-00009.
10
Contribution of phagocytic cells and bacteria to the accumulation of technetium-99m labelled polyclonal human immunoglobulin at sites of inflammation.吞噬细胞和细菌对炎症部位锝-99m标记的多克隆人免疫球蛋白蓄积的作用。
Eur J Nucl Med. 1995 Jul;22(7):638-44. doi: 10.1007/BF01254565.

引用本文的文献

1
Technetium-99m Tricarbonyl Labeled a Broad-spectrum Quinolone as a Specific Imaging Agent in Infection Diseases.锝-99m三羰基标记的广谱喹诺酮作为感染性疾病的特异性显像剂
Iran J Pharm Res. 2017 Spring;16(2):611-618.
2
The uptake mechanisms of inflammation- and infection-localizing agents.炎症和感染定位剂的摄取机制。
Eur J Nucl Med. 1996 Apr;23(4):459-65. doi: 10.1007/BF01247377.
3
Optimized localization of bacterial infections with technetium-99m labelled human immunoglobulin after protein charge selection.蛋白质电荷选择后，用锝-99m标记的人免疫球蛋白对细菌感染进行优化定位。

本文引用的文献

1
"Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.来自金黄色葡萄球菌的“蛋白A”。I. 与人γ-球蛋白的假免疫反应。
J Immunol. 1966 Dec;97(6):822-7.
2
Detection of acute inflammation with 111In-labeled nonspecific polyclonal IgG.用铟-111标记的非特异性多克隆免疫球蛋白检测急性炎症
Semin Nucl Med. 1988 Oct;18(4):335-44. doi: 10.1016/s0001-2998(88)80042-4.
3
Influence of etoposide and cyclophosphamide on the efficacy of cloxacillin and erythromycin in an experimental staphylococcal infection.依托泊苷和环磷酰胺对氯唑西林和红霉素治疗实验性葡萄球菌感染疗效的影响。
Eur J Nucl Med. 1994 Oct;21(10):1135-40. doi: 10.1007/BF00181070.
4
Contribution of phagocytic cells and bacteria to the accumulation of technetium-99m labelled polyclonal human immunoglobulin at sites of inflammation.吞噬细胞和细菌对炎症部位锝-99m标记的多克隆人免疫球蛋白蓄积的作用。
Eur J Nucl Med. 1995 Jul;22(7):638-44. doi: 10.1007/BF01254565.
Antimicrob Agents Chemother. 1989 Jun;33(6):980-2. doi: 10.1128/AAC.33.6.980.
4
Radiolabeled, nonspecific, polyclonal human immunoglobulin in the detection of focal inflammation by scintigraphy: comparison with gallium-67 citrate and technetium-99m-labeled albumin.放射性标记的非特异性多克隆人免疫球蛋白在闪烁扫描术检测局灶性炎症中的应用：与枸橼酸镓-67和锝-99m标记白蛋白的比较
J Nucl Med. 1989 Mar;30(3):385-9.
5
Quo vadis radioimmune imaging.
J Nucl Med. 1989 Nov;30(11):1911-5.
6
Autoradiographic method for quantitation of radiolabeled proteins in tissues using indium-111.
J Nucl Med. 1989 Sep;30(9):1538-45.
7
Localization of Fc and Fab fragments of nonspecific polyclonal IgG at focal sites of inflammation.非特异性多克隆IgG的Fc和Fab片段在炎症灶处的定位。
J Nucl Med. 1990 Jul;31(7):1199-205.
8
99mTc-labelled polyclonal human immunoglobulin for localization of inflammatory sites--early in vitro-results.用于炎症部位定位的99mTc标记多克隆人免疫球蛋白——早期体外实验结果
Nuklearmedizin. 1990 Mar;29(2):54-8.
9
Scintigraphic detection of bone and joint infections with indium-111-labeled nonspecific polyclonal human immunoglobulin G.用铟 - 111标记的非特异性多克隆人免疫球蛋白G进行骨与关节感染的闪烁扫描检测。
J Nucl Med. 1990 Apr;31(4):403-12.
10
99mTc-human immunoglobulin (HIG)--first results of a new agent for the localization of infection and inflammation.99m锝标记人免疫球蛋白（HIG）——一种用于感染和炎症定位的新型制剂的初步结果。
Eur J Nucl Med. 1990;16(8-10):649-55. doi: 10.1007/BF00998164.