Chang Y F, Lauderdale T L, Lee W Y, Shin S J, Jacobson R H, Appel M J, Lein D H
Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY.
FEMS Microbiol Lett. 1993 May 15;109(2-3):297-301. doi: 10.1016/0378-1097(93)90036-2.
Outer surface protein A (OspA) is encoded by the ospA gene from Borrelia burgdorferi. This protein induces immunity against infection in mice. The cloning and expression of OspA in Escherichia coli have been previously described, but the secretion of OspA into culture media in E. coli has not yet been reported. In this report we demonstrate that a chimeric OspA protein was secreted into culture media by E. coli when it also harbors the hemolysin secretion genes hlyBD. The OspA fusion protein was also overexpressed from a T7 promoter and purified by immobilized metal ion chromatography. This was possible because the fusion protein contains six histidyl residues in its N-terminus.
外表面蛋白A（OspA）由伯氏疏螺旋体的ospA基因编码。该蛋白可诱导小鼠产生抗感染免疫力。此前已有关于OspA在大肠杆菌中的克隆和表达的描述，但尚未有关于OspA在大肠杆菌中分泌到培养基中的报道。在本报告中，我们证明，当大肠杆菌同时携带溶血素分泌基因hlyBD时，一种嵌合OspA蛋白会分泌到培养基中。OspA融合蛋白也从T7启动子过表达，并通过固定化金属离子色谱法进行纯化。这是可行的，因为融合蛋白在其N端含有六个组氨酸残基。
