Scott R C, Carmichael N G, Huckle K R, Needham D, Savage T
Zeneca Central Toxicology Laboratory, UK.
Food Chem Toxicol. 1993 Jul;31(7):523-9. doi: 10.1016/0278-6915(93)90113-d.
The quantitation of percutaneous absorption of pesticides is required as part of the registration, re-registration or hazard assessment process. There is a paucity of regulatory guidelines in this area. This paper presents three protocols that can be used to quantitate percutaneous absorption, primarily as a result of continuous skin exposure over a period equivalent to a working day (8 hr). A rat in vivo protocol, an in vitro protocol and a human in vivo protocol are described. None of these protocols is considered to be ideal and/or to represent a preferred method. The final choice of protocol must take into account the toxicity and physicochemical properties of the test molecule as well as cost and resource/technical ability. Nevertheless, the protocols described allow percutaneous absorption to be quantitated, and it is believed that, if adopted, they will prove useful in the regulatory and research areas for the acquisition of data under standard defined conditions.
作为农药登记、重新登记或危害评估过程的一部分，需要对农药的经皮吸收进行定量。该领域的监管指南很少。本文介绍了三种可用于定量经皮吸收的方案，主要是由于在相当于一个工作日（8小时）的时间段内持续皮肤接触所致。描述了大鼠体内方案、体外方案和人体体内方案。这些方案均不被认为是理想的和/或代表首选方法。方案的最终选择必须考虑测试分子的毒性和物理化学性质以及成本和资源/技术能力。然而，所描述的方案能够对经皮吸收进行定量，并且据信，如果采用这些方案，它们将在监管和研究领域证明对于在标准定义条件下获取数据很有用。
