Moran C
Department of Animal Science, University of Sydney, NSW, Australia.
J Hered. 1993 Jul-Aug;84(4):274-80. doi: 10.1093/oxfordjournals.jhered.a111339.
Separate databases were made for all pig and chicken sequences in the Genbank database, which were then systematically searched for all possible mononucleotide, dinucleotide, trinucleotide, and tetranucleotide repeats of about 20 bp or greater in length. A total of 13% of pig genes and 10% of chicken genes contained one or more of these microsatellite-like repeats. There was a considerable difference in the frequency of dinucleotide repeats between pigs, where they comprised 30% of the repeats found, and chickens, where they comprised only 12%. The AC/GT category displayed the greatest difference: seven cases were found from 181 genes in pig (3.9%), whereas only five were found from 531 genes in chicken (0.9%). There appears to be a substantial difference in the spectrum of microsatellites between pigs and chickens. Primer sequences for amplifying these microsatellites are presented.
在Genbank数据库中为所有猪和鸡的序列建立了单独的数据库，然后系统地搜索长度约20 bp或更长的所有可能的单核苷酸、二核苷酸、三核苷酸和四核苷酸重复序列。总共13%的猪基因和10%的鸡基因包含一个或多个这些微卫星样重复序列。猪中二核苷酸重复序列的频率与鸡有相当大的差异，在猪中，二核苷酸重复序列占所发现重复序列的30%，而在鸡中仅占12%。AC/GT类别差异最大：在猪的181个基因中发现了7个（3.9%），而在鸡的531个基因中仅发现了5个（0.9%）。猪和鸡之间微卫星的谱似乎有很大差异。本文给出了扩增这些微卫星的引物序列。
