Lagercrantz U, Ellegren H, Andersson L
Department of Plant Breeding, Swedish University of Agricultural Sciences, Uppsala.
Nucleic Acids Res. 1993 Mar 11;21(5):1111-5. doi: 10.1093/nar/21.5.1111.
The abundance of different simple sequence motifs in plants was accessed through data base searches of DNA sequences and quantitative hybridization with synthetic dinucleotide repeats. Database searches indicated that microsatellites are five times less abundant in the genomes of plants than in mammals. The most common plant repeat motif was AA/TT followed by AT/TA and CT/GA. This group comprised about 75% of all microsatellites with a length of more than 6 repeats. The GT/CA motif being the most abundant dinucleotide repeat in mammals was found to be considerably less frequent in plants. To address the question if plant simple repeat sequences are variable as in mammals, (GT)n and (CT)n microsatellites were isolated from B.napus. Five loci were investigated by PCR-analysis and amplified products were obtained for all microsatellites from B. oleracea, B.napus and B.rapa DNA, but only for one primer pair from B.nigra. Polymorphism was detected for all microsatellites.
通过对DNA序列进行数据库搜索以及与合成二核苷酸重复序列进行定量杂交，来了解植物中不同简单序列基序的丰度。数据库搜索表明，植物基因组中微卫星的丰度比哺乳动物基因组中的低五倍。最常见的植物重复基序是AA/TT，其次是AT/TA和CT/GA。这一组约占所有长度超过6个重复序列的微卫星的75%。在哺乳动物中最丰富的二核苷酸重复基序GT/CA在植物中出现的频率要低得多。为了探讨植物简单重复序列是否像哺乳动物中的那样具有变异性，从甘蓝型油菜中分离出了(GT)n和(CT)n微卫星。通过PCR分析研究了五个位点，从甘蓝、甘蓝型油菜和白菜型油菜的DNA中获得了所有微卫星的扩增产物，但从黑芥DNA中仅获得了一对引物的扩增产物。所有微卫星均检测到多态性。
