Yamaguchi S, Yoshioka T, Utsunomiya M, Koide T, Osafune M, Okuyama A, Sonoda T
Department of Urology, Osaka University Medical School, Japan.
Urol Res. 1993 May;21(3):187-92. doi: 10.1007/BF00590035.
The nature of the soluble stone matrix and its possible role in urinary stone formation was studied. For this purpose we performed two-dimensional cellulose acetate membrane electrophoresis of the glycosaminoglycans (GAGs) which were contained in the soluble stone matrix, substances adsorbed onto calcium oxalate crystals in vitro (crystal surface binding substances, CSBS) and urinary macromolecules (UMMs). The main GAG in the soluble stone matrix and CSBS was found to be heparan sulfate, whereas the UMMs contained various GAGs usually seen in urine. An inhibition assay showed the soluble stone matrix to have the strongest inhibitory activity among these macromolecular substances when inhibitory activity was expressed in terms of uronic acid concentration. It is suggested that the main GAG in the soluble stone matrix consists of heparan sulfate, which has a strong inhibitory activity on calcium oxalate crystal growth and aggregation and constitutes part of the CSBS.
研究了可溶性结石基质的性质及其在尿路结石形成中的可能作用。为此，我们对可溶性结石基质中所含的糖胺聚糖（GAGs）、体外吸附在草酸钙晶体上的物质（晶体表面结合物质，CSBS）和尿大分子（UMMs）进行了二维醋酸纤维素膜电泳。结果发现，可溶性结石基质和CSBS中的主要GAG是硫酸乙酰肝素，而UMMs则含有尿液中常见的各种GAG。抑制试验表明，当以糖醛酸浓度表示抑制活性时，可溶性结石基质在这些大分子物质中具有最强的抑制活性。提示可溶性结石基质中的主要GAG由硫酸乙酰肝素组成，其对草酸钙晶体的生长和聚集具有较强的抑制活性，并构成CSBS的一部分。
