Scherer G, Hobom G, Kössel H
Nature. 1977 Jan 13;265(5590):117-21. doi: 10.1038/265117a0.
The sequence of a 123 base pair HpaII restriction fragment of bacteriophage lamdba DNA has been determined by the dimethylsulphate-hydrazine technique. Of this fragment 65 nucleotide pairs are transcribed into the 5' proximal part of the oop RNA. The remaining 58 nucleotide pairs preceding the start point of transcription show homologies to the lamdbaL and lamdbapR promoter regions, and are concluded to contain the lamdbapo promoter sequence.
通过硫酸二甲酯-肼技术确定了噬菌体λDNA的一个123个碱基对的HpaII限制性片段的序列。该片段中的65个核苷酸对被转录到oop RNA的5'近端部分。转录起始点之前的其余58个核苷酸对与λL和λpR启动子区域具有同源性,并被推断包含λpO启动子序列。