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噬菌体fd启动子功能所必需的DNA区域。

DNA regions essential for the function of a bacteriophage fd promoter.

作者信息

Okamoto T, Sugimoto K, Sugisaki H, Takanami M

出版信息

Nucleic Acids Res. 1977 Jul;4(7):2213-22. doi: 10.1093/nar/4.7.2213.

DOI:10.1093/nar/4.7.2213
PMID:909770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342560/
Abstract

The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiation site). The promoter was cleaved into two fragments by R-Hha I and each promoter fragment was joined to DNA fragments derived from other regions. Ligation of the TATAAT-containing fragment to any of the DNA fragments examined resulted in recovery of promoter function. The results suggest for this type of promoter that no unique sequence is necessary upstream from the R-Hha I cleavage site although a contiguous DNA chain must be present in this area.

摘要

噬菌体fd主要外壳蛋白基因的启动子包含一个独特序列。在对应于Pribnow框的非转录区域中有TATAAT。一个破坏功能的R-Hha I切割位点位于TATAAT序列上游五对处（RNA起始位点上游十五个碱基对）。启动子被R-Hha I切割成两个片段，每个启动子片段都与来自其他区域的DNA片段连接。将含有TATAAT的片段与所检测的任何DNA片段连接都会恢复启动子功能。结果表明，对于这种类型的启动子，尽管该区域必须存在连续的DNA链，但R-Hha I切割位点上游不需要独特序列。

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1
DNA regions essential for the function of a bacteriophage fd promoter.噬菌体fd启动子功能所必需的DNA区域。
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2
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3
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T4 DNA聚合酶
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