García J A, Cervera M T, Riechmann J L, López-Otín C
Centro de Biología Molecular (CSIC-UAM)-Centro Nacional de Biotecnología, Campus de la Universidad Autónoma de Madrid, Spain.
Plant Mol Biol. 1993 Jul;22(4):697-701. doi: 10.1007/BF00047410.
The effect of different protease inhibitors on the proteolytic processing of the plum pox potyvirus (PPV) polyprotein has been analyzed. Human cystatin C, an inhibitor of cysteine proteases, interfered with the autoprocessing of the viral papain-like cysteine protease HCPro. Unexpectedly, it also had an inhibitory effect on the autocatalytic cleavage of the Nla protease which, although it has a Cys residue in its active center, has been described as structurally related to serine proteases. Other protease inhibitors tested had no effect on any of the cleavage events analyzed.
已分析了不同蛋白酶抑制剂对李痘马铃薯Y病毒（PPV）多聚蛋白蛋白水解加工的影响。人胱抑素C是一种半胱氨酸蛋白酶抑制剂，它干扰了病毒木瓜蛋白酶样半胱氨酸蛋白酶HCPro的自我加工。出乎意料的是，它对Nla蛋白酶的自催化切割也有抑制作用，尽管Nla蛋白酶在其活性中心有一个半胱氨酸残基，但已被描述为在结构上与丝氨酸蛋白酶相关。所测试的其他蛋白酶抑制剂对所分析的任何切割事件均无影响。
