凝血因子 XIIIB 分子表型免疫检测的另一种方法。

An alternative modality for the immunodetection of the molecular phenotypes of coagulation factor XIIIB.

作者信息

Cameselle F D, Caeiro B, Riveiro A

机构信息

Departamento de Antropoloxía, Facultade de Bioloxía, Universidade de Santiago, España.

出版信息

Hum Hered. 1993 Jul-Aug;43(4):257-60. doi: 10.1159/000154140.

DOI:10.1159/000154140

PMID:8344671

Abstract

A new technical modality for the typing of coagulation factor XIIIB (F13B) is described. The determination of F13B phenotypes was carried out from desyalized plasma samples by means of polyacrylamide gel isoelectric focusing (pH range 6-8) followed by immunofixation-silver stain. This method combines high sensitivity, low expenditure, and a single methodology. A genetic analysis on F13B phenotypes in the Galician population is also discussed.

摘要

本文描述了一种用于凝血因子 XIIIB（F13B）分型的新技术方法。通过聚丙烯酰胺凝胶等电聚焦（pH范围6 - 8），随后进行免疫固定 - 银染，从去唾液酸化血浆样本中确定F13B表型。该方法具有高灵敏度、低成本且方法单一的特点。本文还讨论了加利西亚人群中F13B表型的遗传分析。