Sakaguchi T, Sakaguchi S, Nakamura I, Kudo Y
Department of Hygiene, St. Marianna University School of Medicine, Kawasaki, Japan.
J Toxicol Environ Health. 1993 Aug;39(4):517-26. doi: 10.1080/15287399309531768.
A 7BeCl2 solution containing 0.5 micrograms Be per mouse was injected subcutaneously, intraperitoneally, intramuscularly, intrathoracically, and intravenously, and distribution was observed for periods up to 1 wk. 7Be was excreted more rapidly following intravenous injection than by the other routes of injection. The amount of Be found in the liver or the spleen was substantial at 1 d after intraperitoneal injection. It increased more in the spleen at 7 d after either intraperitoneal or intrathoracic injection. On the other hand, the amounts of Be stayed almost constant in the kidneys, by the various routes of injection. When injected intrathoracically, the amounts of Be in the heart and the lung were greater than when administered by the other routes of injection. The amounts of Be in the femurs of mice administered by these routes of injection, except with intravenous injection, were greater than in the other organs. The percentage of 7Be in the mineralized bone was 90% of that of 7Be in the femurs when injected intraperitoneally or intrathoracically. However, the ratio of Be in the mineralized bone to that in the bone marrow was 3 to 2. Beryllium had thus a closer affinity for the femurs than for the other organs investigated, with the different modes of administration used. The amount of Be in the entire skeleton was estimated to be substantial. Within the limitations of 1 wk of exposure, the skeleton would appear to be a critical organ. This would suggest that osteosarcomas may occur following administration of Be to laboratory animals for a long-term period.
向每只小鼠皮下、腹腔内、肌肉内、胸腔内和静脉内注射含0.5微克铍的7BeCl2溶液，并观察长达1周的分布情况。静脉注射后，7Be的排泄速度比其他注射途径更快。腹腔注射后1天，在肝脏或脾脏中发现的铍量相当可观。腹腔或胸腔注射后7天，脾脏中的铍量增加更多。另一方面，通过各种注射途径，肾脏中的铍量几乎保持恒定。胸腔内注射时，心脏和肺中的铍量比其他注射途径给药时更多。通过这些注射途径给药的小鼠股骨中的铍量，除静脉注射外，均高于其他器官。腹腔或胸腔注射时，矿化骨中7Be的百分比是股骨中7Be的90%。然而，矿化骨中的铍与骨髓中的铍之比为3比2。因此，在使用不同给药方式时，铍对股骨的亲和力比对其他研究器官的亲和力更强。估计整个骨骼中的铍量相当可观。在1周暴露的限制范围内，骨骼似乎是一个关键器官。这表明长期给实验动物施用铍后可能会发生骨肉瘤。
