Scott T R, Savage M L, Olah I
Department of Poultry Science, Clemson University, South Carolina 29634-0379.
Poult Sci. 1993 Jul;72(7):1273-9. doi: 10.3382/ps.0721273.
The chicken Harderian gland (HG) is densely populated in its subepithelial spaces with plasma cells (PC). These immune cells produce and secrete Ig of the IgA, IgG, and IgM classes. Such Ig secretion into the tears affords the upper respiratory tract with protective antibodies. The immunological role of the HG is quite interesting; yet this gland is a site of unusual PC proliferation. Studies of the gland utilizing bromodeoxyuridine (BrdUrd) incorporation into DNA and propidium iodide (PI) staining of PC DNA have verified previous suggestions in the literature that PC of the chicken HG proliferate. Both isolated PC suspensions and frozen sections of the HG from chicks aged 6 to 9 wk reveal that BrdUrd is incorporated into PC DNA. Furthermore, flow cytometric analysis of PI-stained PC indicates a relatively high percentage of PC in S phase of the cell cycle. Continued studies are examining possible mechanisms controlling proliferation and differentiation of PC in the HG. It is believed that the stromal elements of the HG produce and secrete a factor(s) that influences PC proliferation and differentiation. Isolation and characterization of this influencing factor(s) will allow for the possible systemic application of the factor(s) for enhancement of immune responses.
鸡的哈德氏腺（HG）上皮下间隙中密集分布着浆细胞（PC）。这些免疫细胞产生并分泌IgA、IgG和IgM类别的免疫球蛋白。这种免疫球蛋白分泌到泪液中，为上呼吸道提供保护性抗体。哈德氏腺的免疫作用相当有趣；然而，该腺体是浆细胞异常增殖的部位。利用溴脱氧尿苷（BrdUrd）掺入DNA以及碘化丙啶（PI）对浆细胞DNA进行染色对该腺体进行的研究，证实了文献中先前的观点，即鸡哈德氏腺的浆细胞会增殖。对6至9周龄雏鸡分离的浆细胞悬液和哈德氏腺冰冻切片的研究均显示，BrdUrd掺入了浆细胞DNA。此外，对PI染色的浆细胞进行流式细胞术分析表明，处于细胞周期S期的浆细胞比例相对较高。后续研究正在探讨控制哈德氏腺浆细胞增殖和分化的可能机制。据信，哈德氏腺的基质成分产生并分泌一种影响浆细胞增殖和分化的因子。分离并鉴定这种影响因子，可能会使其在全身应用以增强免疫反应。
