Gallego M, Glick B
Poultry Science Department, Clemson University, SC 29634.
Dev Comp Immunol. 1988 Winter;12(1):157-66. doi: 10.1016/0145-305x(88)90033-x.
The largest concentration of plasmacytic cells within avian lymphomyeloid tissue appears in the Harderian Gland (HG). Mature plasma cells were the least frequent cell at day old and the most frequent by 5-weeks of age. Failure of phytohemagglutinin-M to stimulate blasting of HG cells may reflect the low number of T cells in the HG. On the other hand, the uptake of tritiated thymidine by HG cells after short term culture in complete medium was significantly greater than that of splenic cells. The proliferating cells were the plasmablast, and mature plasma cell. Our data demonstrated that labeled B-cells, administered intravenously to 2- or 6-week old syngeneic or allogeneic chickens, did not migrate to the HG. The origin of HG plasma cells may be B-cells which entered prior to 2 weeks of age or the HG may possess a special microenvironment which allowed stem cell differentiation. The maintenance of a high concentration of plasma cells in the HG in the presence of low levels of B-cells may reflect the long-lived nature of the resident B-cell and/or the inherent proliferative capacity of the HG plasmacytic cell.
禽类淋巴髓样组织中浆细胞的最大聚集出现在哈德氏腺（HG）。成熟浆细胞在雏鸡日龄时是最不常见的细胞，而在5周龄时是最常见的细胞。植物血凝素-M未能刺激HG细胞的增殖，这可能反映了HG中T细胞数量较少。另一方面，在完全培养基中短期培养后，HG细胞对氚标记胸腺嘧啶核苷的摄取明显高于脾细胞。增殖细胞是成浆细胞和成熟浆细胞。我们的数据表明，静脉注射到2周龄或6周龄同基因或异基因鸡体内的标记B细胞不会迁移到HG。HG浆细胞的来源可能是在2周龄之前进入的B细胞，或者HG可能拥有允许干细胞分化的特殊微环境。在B细胞水平较低的情况下，HG中浆细胞的高浓度维持可能反映了驻留B细胞的长寿特性和/或HG浆细胞固有的增殖能力。
