Murata S, Takasaki N, Saitoh M, Okada N
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):6995-9. doi: 10.1073/pnas.90.15.6995.
Several subfamilies of the salmonid Hpa I short interspersed element (SINE) family were isolated from salmonid genomes and were sequenced. For each genomic locus that represented the subfamily, amplification by PCR of the orthologous loci in the 12 fish allowed us to determine the order of branching of the Pacific salmonid species. The deduced phylogeny suggests three evolutionary lines, namely, a line of chum salmon, pink salmon, and kokanee; a line of coho salmon and chinook salmon; and a line of steelhead trout. Our data also support a change in the phylogenetic assignment of steelhead trout from Salmo to Oncorhynchus. We present here an extensive phylogenetic tree constructed from an analysis of differential insertion of SINEs, and we propose that SINE insertion analysis is one of the best available methods for clarifying the order of divergence of closely related species.
从鲑科基因组中分离出鲑科Hpa I短散在元件（SINE）家族的几个亚家族并进行了测序。对于代表该亚家族的每个基因组位点，通过PCR扩增12种鱼类中的直系同源位点，使我们能够确定太平洋鲑科物种的分支顺序。推导的系统发育表明有三条进化线，即：大麻哈鱼、细鳞大麻哈鱼和红大麻哈鱼线；银大麻哈鱼和大鳞大麻哈鱼线；以及虹鳟鱼线。我们的数据还支持将虹鳟鱼的系统发育归属从鲑属更改为大麻哈鱼属。我们在此展示了一个通过对SINEs差异插入分析构建的广泛系统发育树，并提出SINE插入分析是阐明近缘物种分化顺序的最佳可用方法之一。
