Connor R I, Notermans D W, Mohri H, Cao Y, Ho D D
Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016.
AIDS Res Hum Retroviruses. 1993 Jun;9(6):541-6. doi: 10.1089/aid.1993.9.541.
Twenty putative clones of HIV-1, derived by biological cloning from two different individuals, were characterized in vitro for functional activity and genotypic diversity. Many of the biological clones isolated by this method were genetically distinct, and could be further distinguished by differences in replication kinetics, host cell range, and/or cytopathicity. Our findings indicate that biological cloning by limiting dilution cultures is an effective method for isolating replication-competent, functionally diverse quasispecies of HIV-1.
