Sánchez-Arroyos R, Gaztelu J M, Zaplana J, Dajas F, García-Austt E
Depto de Investigación, Hospital Ramón y Cajal, Madrid, Spain.
Brain Res Bull. 1993;32(2):171-8. doi: 10.1016/0361-9230(93)90071-i.
Hippocampal and entorhinal cortex glucose metabolism were studied by 14C-2-deoxyglucose (2-DG) autoradiography in anesthetized rats with and without continuous theta rhythm (theta). 2-Deoxyglucose changes in specific cytoarchitectonic regions were precisely assessed by n innovative approach. In the absence of theta there were areas with a higher glucose metabolism corresponding to neuropile regions at CA3, dentate gyrus, and subiculum, while the cellular layers always showed lower values. In the presence of theta, provoked by intraventricular injections of anticholinesterases (i.e., physostigmine) or curarimimetics (i.e., d-tubocurarine), 2-DG uptake showed two opposite significant changes in relation to controls: a) it increased in the outer zone of the molecular layer (inner blade) of the dentate gyrus, and in the stratum lacunosum-moleculare of CA3, suggesting an increase in perforant path input during theta rhythm; b) it decreased in the hilar dentate region. This noteworthy decrease in metabolic activity probably reflects an hilar inhibition by local circuits during theta rhythm generation.
通过14C-2-脱氧葡萄糖（2-DG）放射自显影技术，对有无持续θ节律（theta）的麻醉大鼠的海马和内嗅皮质葡萄糖代谢进行了研究。采用一种创新方法精确评估了特定细胞构筑区域的2-脱氧葡萄糖变化。在无θ节律时，存在葡萄糖代谢较高的区域，对应于CA3、齿状回和海马下托的神经毡区域，而细胞层的代谢值始终较低。在通过脑室内注射抗胆碱酯酶（即毒扁豆碱）或箭毒样物质（即d-筒箭毒碱）引发θ节律时，与对照组相比，2-DG摄取显示出两个相反的显著变化：a）在齿状回分子层（内叶片）的外层区域以及CA3的腔隙-分子层中增加，表明在θ节律期间穿通通路输入增加；b）在齿状回门区减少。这种代谢活性的显著降低可能反映了在θ节律产生过程中局部回路对门区的抑制作用。
