Nakajima N, Utsunomiya T, Kobayashi M, Herndon D N, Pollard R B, Suzuki F
Department of Internal Medicine, University of Texas Medical Branch, Galveston, USA.
Burns. 1996 Dec;22(8):612-7. doi: 10.1016/s0305-4179(96)00053-8.
Glycyrrhizin (GR), an active component of licorice roots, has been previously shown to induce the generation of anti-type 2 T cells (anti-BI2T cells), which are able to counteract the activity of burn-induced CD8+ type 2 T cells (BI2T cells), in thermally injured and normal mice. In the present study, anti-BI2T cells were generated in vitro in cultures of spleen cells stimulated with GR. Anti-BI2T cells were induced in vitro when splenic mononuclear cells (SMNC) were stimulated in vitro for 24 to 72 h with 0.1-10 micrograms/ml of GR. Anti-BI2T cell activity was detected when suppressor macrophages (M phi) were depleted from SMNC after stimulation with GR. However, the GR-stimulated generation of anti-BI2T cells required the participation of M phi or their sonicated fractions. Anti-BI2T cells induced in vitro by GR were identified as Vicia villosa lectin adherent IFN7 producing-CD4+ T cells lacking the ability to produce IL-2, IL-4 or IL-10. These results indicate that anti-BI2T cells are generated by GR in vitro. M phi are not only necessary for the generation of anti-BI2T cells induced by GR but also inhibit their activity.
甘草甜素（GR）是甘草根的一种活性成分，先前已证明它能在热损伤小鼠和正常小鼠中诱导产生抗2型T细胞（抗BI2T细胞），这些细胞能够对抗烧伤诱导的CD8 + 2型T细胞（BI2T细胞）的活性。在本研究中，抗BI2T细胞是在GR刺激的脾细胞培养物中体外产生的。当脾单核细胞（SMNC）用0.1 - 10微克/毫升的GR体外刺激24至72小时时，可在体外诱导产生抗BI2T细胞。在用GR刺激后，当从SMNC中去除抑制性巨噬细胞（M phi）时，可检测到抗BI2T细胞活性。然而，GR刺激产生抗BI2T细胞需要M phi或其超声破碎产物的参与。GR体外诱导的抗BI2T细胞被鉴定为缺乏产生IL - 2、IL - 4或IL - 10能力的野豌豆凝集素粘附的IFNγ产生-CD4 + T细胞。这些结果表明GR在体外可产生抗BI2T细胞。M phi不仅是GR诱导产生抗BI2T细胞所必需的，而且还抑制其活性。
