Perez F, Rochd T, Lodter J P, Calas P, Michel G
UFR Odontologie, Toulouse, France.
Oral Surg Oral Med Oral Pathol. 1993 Jul;76(1):97-103. doi: 10.1016/0030-4220(93)90302-k.
The purpose of this study was to assess in vitro migration of the following three bacterial species into dentinal tubules: Streptococcus sanguis, Actinomyces naeslundii and Prevotella intermedia. Twenty-seven bovine incisors were randomly divided into three groups. Only the root dentin was used for the experiment. Within each group, the nine incisors were sectioned into 36 dentin blocks that were incubated with one of the tested bacterial suspensions. Twelve samples were taken after 10, 20, and 28 days. Half the samples were observed with scanning electron microscopy and the others with light microscopy after standard histologic procedures, and the depth of bacterial penetration was measured. Analysis of the examinations showed that whatever the technique used, only one strain on this experimental model migrated into dentinal tubules. S. sanguis was observed at a depth of 792 microns, but no migration was observed for either A.naeslundii or P.intermedia. The differences in migration of the bacteria tested appear to be related to their morphologic factors and cellular arrangement.
血链球菌、内氏放线菌和中间普氏菌。将27颗牛切牙随机分为三组。实验仅使用牙根牙本质。每组内，将9颗切牙切成36个牙本质块，与一种受试细菌悬液一起孵育。在10天、20天和28天后各取12个样本。一半样本经标准组织学程序处理后用扫描电子显微镜观察，另一半用光学显微镜观察，并测量细菌侵入的深度。检查分析表明，无论使用何种技术，在该实验模型中只有一种菌株迁移到了牙本质小管内。观察到血链球菌侵入深度达792微米，但未观察到内氏放线菌或中间普氏菌的迁移。受试细菌迁移情况的差异似乎与其形态学因素和细胞排列有关。
