Perez F, Calas P, de Falguerolles A, Maurette A
Service d'Odontologie Conservatrice-Endodontie, Faculté de Chirurgie Dentaire, Toulouse, France.
J Endod. 1993 Jun;19(6):297-301. doi: 10.1016/s0099-2399(06)80460-7.
The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The aim of this study was to study the in vitro penetration of root dentinal tubules by bacteria. Bovine dentin was inoculated with a strain of Streptococcus sanguis, NCTC 7863; samples were taken at 10, 20, and 28 days, and bacterial penetration of the tubules was demonstrated using two techniques, scanning electron microscopy and light microscopy after Brown and Brenn staining. The results showed that S. sanguis can penetrate deep inside the tubules (792 microns). The maturity of the dentin blocks seems to be a major factor in tubule penetration.
根管系统中细菌的持续存在常常导致治疗失败。本研究的目的是研究细菌在体外对牙本质小管的渗透情况。用一株血链球菌NCTC 7863接种牛牙本质；在第10、20和28天取样，并使用扫描电子显微镜和Brown和Brenn染色后的光学显微镜这两种技术来证明细菌对小管的渗透。结果表明，血链球菌能够深入小管内部（792微米）。牙本质块的成熟度似乎是小管渗透的一个主要因素。
