Tasanarong Parinda, Dechatiwongse Na Ayudhya Thaweephol, Techanitiswad Tipawan, Koontongkaew Sittichai
Faculty of Dentistry, Thammasat University, Rangsit Campus, Pathum Thani, Thailand.
Medicinal Herb Research Unit for Asthma, Thammasat University, Rangsit Campus, Pathum Thani, Thailand.
J Dent Sci. 2016 Dec;11(4):419-426. doi: 10.1016/j.jds.2016.06.006. Epub 2016 Aug 10.
BACKGROUND/PURPOSE: The 3Mix-MP formulation (a mixture of metronidazole, ciprofloxacin, and minocycline; macrogol and propylene glycol) has been used to kill residual bacteria in dentin caries. This study aimed to investigate the dentin disinfection and cytotoxicity of a novel zinc oxide (ZnO) based medicament, Z-Mix.
ZMix was prepared as a prefilled syringe of materials containing mainly ZnO, incorporated with amoxicillin, ciprofloxacin, and metronidazole (1 g% of each antibiotic). Drug penetration was measured at 24 hours and 72 hours. , , or were inoculated into dentinal tubules for 30 days and were then subjected to Z-Mix or 0.2% chlorhexidine (CHX) for 48 hours. Viable bacteria in the dentin were determined using fluorescence staining. Their cytotoxicity against human dental pulp cells was assessed using an MTT assay.
ZMix obviously diffused into dentinal tubules and the root apex, compared to the 3Mix-MP (P < 0.05). Fluorescence staining demonstrated a reduction of viable bacteria at 100 μm and 500 μm below infected cavities after treatment with Z-mix or CHX for 48 hours. Live and dead bacteria ratios indicated that Z-Mix exhibited markedly antimicrobial effects on inoculated bacteria in dentin samples (P < 0.05). There was no significant difference in the antimicrobial property between Z-Mix and CHX (P > 0.05). An acceptable level of cytotoxicity was observed in Z-Mix and its ingredients.
Z-Mix, a soft shapeable paste containing a mixture of three antibiotics, is successfully. It can penetrate to the root apex and exhibits antimicrobial properties.
背景/目的：3Mix-MP配方（甲硝唑、环丙沙星和米诺环素的混合物；聚乙二醇和丙二醇）已被用于杀灭牙本质龋中的残留细菌。本研究旨在调查一种新型的基于氧化锌（ZnO）的药物Z-Mix的牙本质消毒和细胞毒性。
将Z-Mix制备成预填充注射器剂型，其材料主要包含ZnO，并加入阿莫西林、环丙沙星和甲硝唑（每种抗生素含量为1g%）。在24小时和72小时测量药物渗透情况。将 、 或 接种到牙本质小管中30天，然后用Z-Mix或0.2%氯己定（CHX）处理48小时。使用荧光染色法测定牙本质中的活菌。使用MTT法评估其对人牙髓细胞的细胞毒性。
与3Mix-MP相比，Z-Mix明显扩散到牙本质小管和根尖（P < 0.05）。荧光染色显示，用Z-Mix或CHX处理48小时后，感染洞下方100μm和500μm处的活菌数量减少。活菌与死菌比例表明，Z-Mix对牙本质样本中接种的细菌具有显著的抗菌作用（P < 0.05）。Z-Mix和CHX之间的抗菌性能没有显著差异（P > 0.05）。在Z-Mix及其成分中观察到可接受水平的细胞毒性。
Z-Mix是一种含有三种抗生素混合物的可塑形软糊剂，已获成功。它可以渗透到根尖并具有抗菌性能。
