Marshak T L, Kleshchinov V N, Brodskiĭ V Ia
Ontogenez. 1993 May-Jun;24(3):53-7.
Cerebellum of rat embryos at day 17 of gestation was transplanted into sensomotor cortex of adult syngenic rats. DNA content in granule and Purkinje cells of the transplant was determined cytophotometrically on day 30 after surgery. It is shown that granule cells are diploid; about 3% Purkinje cells contain hyperdiploid and tetraploid nuclei which corresponds to the content of such cells in the adult cerebellum. The conclusion is drawn that cell hyperploidy does not depend on the functional load but is essential property of the tissue.
将妊娠第17天大鼠胚胎的小脑移植到同基因成年大鼠的感觉运动皮层。术后第30天,用细胞光度法测定移植组织中颗粒细胞和浦肯野细胞的DNA含量。结果表明,颗粒细胞为二倍体;约3%的浦肯野细胞含有超二倍体和四倍体细胞核,这与成年小脑中此类细胞的含量相符。得出的结论是,细胞多倍体不依赖于功能负荷,而是组织的基本特性。