Taussig R, Carlson M
Nucleic Acids Res. 1983 Mar 25;11(6):1943-54. doi: 10.1093/nar/11.6.1943.
The yeast SUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5' and 3' flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within the SUC2 gene but share the same reading frame. The amino acid sequences predicted for the two forms of invertase from the nucleotide sequence are consistent with the properties of the purified enzymes. Potential sites for glycosylation of the secreted invertase are identified.
酵母SUC2基因是转化酶分泌形式和细胞内形式的结构基因。我们已经确定了编码区以及5'和3'侧翼区的核苷酸序列。分泌型转化酶含信号肽前体和细胞内转化酶的编码区在SUC2基因内的不同起始密码子处开始，但具有相同的阅读框。根据核苷酸序列预测的两种形式转化酶的氨基酸序列与纯化酶的特性一致。已确定分泌型转化酶的潜在糖基化位点。
