主要组织相容性复合体I类转录因子H2TF1（nfkb2基因的全长产物）的纯化。
Purification of the major histocompatibility complex class I transcription factor H2TF1. The full-length product of the nfkb2 gene.
作者信息
Potter D A, Larson C J, Eckes P, Schmid R M, Nabel G J, Verdine G L, Sharp P A
机构信息
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Biol Chem. 1993 Sep 5;268(25):18882-90.
Abstract
H2TF1 is a ubiquitous major histocompatibility complex (MHC) class I-specific transcription factor, which binds to the palindromic kappa B enhancer site upstream of MHC class I genes. Here we report that H2TF1 consists of a polypeptide with relative molecular mass 110,000, that corresponds to the predicted 100-kDa product (NF-kappa B2 p100) encoded by the candidate proto-oncogene nfkb2 (lyt-10). H2TF1 was purified by a novel affinity chromatography method and identified as the NF-kappa B2 p100 polypeptide by peptide sequencing as well as by reactivity with a specific antiserum. Purified H2TF1 binds the MHC kappa B site with high affinity (KD = 3 x 10(-11) M), in contrast with previous reports that NF-kappa B2 p100 did not bind DNA.
摘要
H2TF1是一种普遍存在的主要组织相容性复合体（MHC）I类特异性转录因子，它与MHC I类基因上游的回文κB增强子位点结合。在此我们报告，H2TF1由一种相对分子质量为110,000的多肽组成，该多肽对应于候选原癌基因nfkb2（lyt - 10）编码的预测100 kDa产物（NF-κB2 p100）。通过一种新型亲和层析方法纯化了H2TF1，并通过肽序列分析以及与特异性抗血清的反应性将其鉴定为NF-κB2 p100多肽。纯化的H2TF1以高亲和力（KD = 3×10⁻¹¹ M）结合MHC κB位点，这与之前关于NF-κB2 p100不结合DNA的报道相反。
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