Gazzah N, Gharib A, Delton I, Molière P, Durand G, Christon R, Lagarde M, Sarda N
INSERM U. 352, Laboratoire de Chimie Biologique, INSAL, Villeurbanne, France.
J Neurochem. 1993 Sep;61(3):1057-63. doi: 10.1111/j.1471-4159.1993.tb03620.x.
We studied the effect of a diet deficient in n-3 fatty acids on the adenosine-dependent melatonin release from cultured rat pineal gland after stimulation by 5'-N-ethylcarboxamidoadenosine (NECA), an A2 adenosine agonist. Experiments were conducted with 2-month-old rats raised on semipurified diets containing either peanut oil (n-3 deficients) or peanut plus rapeseed oil (controls). The proportion of docosahexaenoic acid (22:6 n-3) in the pineal total lipid fraction and in phosphatidylcholine and phosphatidylethanolamine was significantly decreased in n-3-deficient rats. This was compensated for partially by an increase in 22:4 n-6 and 22:5 n-6 levels. The activity of the cultured rat pineal, in terms of cyclic AMP content and N-acetylserotonin and melatonin release in the medium, was lower after stimulation by 10(-5) mol/L NECA in the group fed peanut oil than in the group fed peanut plus rapeseed oil. The increased ratio of n-6/n-3 fatty acids in pineal total lipids and the major glycerophospholipids (phosphatidylcholine and phosphatidylethanolamine) may have an important influence on the rat pineal responses. The results are discussed in the context of changes in membrane-bound proteins, including enzymes and/or receptors involved in the rat pineal gland function.
我们研究了缺乏n-3脂肪酸的饮食对经A2腺苷激动剂5'-N-乙基羧基酰胺腺苷（NECA）刺激后培养的大鼠松果体中腺苷依赖性褪黑素释放的影响。实验用2个月大的大鼠进行，这些大鼠饲养在含有花生油（n-3缺乏组）或花生油加菜籽油（对照组）的半纯化饮食中。在n-3缺乏的大鼠中，松果体总脂质部分以及磷脂酰胆碱和磷脂酰乙醇胺中二十二碳六烯酸（22:6 n-3）的比例显著降低。这部分被22:4 n-6和22:5 n-6水平的增加所补偿。在用10(-5)mol/L NECA刺激后，喂食花生油组的培养大鼠松果体在环磷酸腺苷含量、N-乙酰血清素和培养基中褪黑素释放方面的活性低于喂食花生油加菜籽油组。松果体总脂质和主要甘油磷脂（磷脂酰胆碱和磷脂酰乙醇胺）中n-6/n-3脂肪酸比例的增加可能对大鼠松果体反应有重要影响。我们在膜结合蛋白变化的背景下讨论了这些结果，膜结合蛋白包括参与大鼠松果体功能的酶和/或受体。
