Hernando F L, Cailliez J C, Trinel P A, Faille C, Mackenzie D W, Poulain D
Dpto. Microbiologia e Immunologia, Fac. Ciencias, Universidad del Pais Vasco, Bilbao, Spain.
J Med Vet Mycol. 1993;31(3):219-26.
Cytoplasmic and cell wall proteins and glycoproteins extracted from Candida albicans germ tubes were screened by Western blotting for their ability to differentiate between the serological responses of patients with candidosis and healthy individuals. Molecules of 114, 74 and 65 kDa were not recognized by any sera. Qualitative differences were observed for responses to proteins and glycoproteins from 29 to 60 kDa. Conversely, only quantitative differences were found to high molecular mass glycoproteins. Their recognition by control sera was invariably associated with reactivity against a 14-18 kDa antigen. However, despite a high level of antibodies against high molecular mass mannoproteins, some patients sera failed to react with the 14-18 kDa antigen, or lost this reactivity during the course of the disease.
通过蛋白质印迹法筛选从白色念珠菌芽管中提取的细胞质、细胞壁蛋白和糖蛋白，以评估其区分念珠菌病患者和健康个体血清学反应的能力。114、74和65 kDa的分子未被任何血清识别。观察到对29至60 kDa的蛋白质和糖蛋白的反应存在定性差异。相反，对于高分子量糖蛋白仅发现定量差异。对照血清对它们的识别总是与针对14 - 18 kDa抗原的反应性相关。然而，尽管针对高分子量甘露糖蛋白的抗体水平很高，但一些患者血清未能与14 - 18 kDa抗原反应，或在疾病过程中失去这种反应性。
