Cailliez J C, Cantelli C, Séguy N, Conti S, Gerloni M, Morace G, Polonelli L
Unité 42, Institut National de la Santé et de la Recherche Médicale, Villeneuve d'Ascq, France.
Mycopathologia. 1994 Jun;126(3):173-7. doi: 10.1007/BF01103772.
A secreted killer toxin was detected through the cell wall of Pichia anomala cells by ultrastructural immunodetection with a specific monoclonal antibody (MAb KT4). MAb KT4 was successively detected by colloidal gold labeled streptavidin and biotinylated anti-mouse F(ab')2 antibodies. The antigenic determinants of the toxin were localized throughout the cytoplasm and the cell wall of killer yeast cells. The Lowicryl K4M-immunogold method gave very satisfactory results and showed that the killer toxin was somewhat concentrated in the yeast cell wall layers before being exported into the medium. In agreement with previous reports, the binding of MAb KT4 suggested that the P. anomala killer toxin secretion did not result from a homogeneous diffusion across the yeast cell wall.
通过用特异性单克隆抗体（MAb KT4）进行超微结构免疫检测，在异常毕赤酵母细胞的细胞壁上检测到一种分泌型杀伤毒素。通过胶体金标记的链霉亲和素和生物素化的抗小鼠F(ab')2抗体相继检测到MAb KT4。毒素的抗原决定簇定位于杀伤酵母细胞的整个细胞质和细胞壁中。低温包埋剂K4M-免疫金法给出了非常令人满意的结果，并表明杀伤毒素在分泌到培养基之前在酵母细胞壁层中有所浓缩。与先前的报道一致，MAb KT4的结合表明异常毕赤酵母杀伤毒素的分泌不是通过在酵母细胞壁上的均匀扩散产生的。
