Burg J G, Knapp F W, Silapanuntakul S
Department of Entomology, University of Kentucky, Lexington 40546.
J Med Entomol. 1993 Jul;30(4):753-7. doi: 10.1093/jmedent/30.4.753.
Studies were conducted to determine optimum replacement intervals, storage times, and vertebrate source of blood used to feed Haematobia irritans (L.) adults in vitro. Feeding success was measured by the total number of larvae produced per female during 10-d experimental periods which combined adult female survival, egg production, and egg hatch. The number of larvae per female was reduced significantly when blood replacement intervals were increased from 24 to 48 h; however, blood replacement intervals could be increased to 48 h without affecting larval production if the blood was agitated continuously. Maximum larval production was obtained when adult flies fed on whole, citrated bovine blood that had been stored at 3 degrees C for 2 wk; however, there was no difference in the number of larvae produced per female when adults were fed blood stored at -20 degrees C for 2-52 wk. Significantly more larvae were produced when adults fed on citrated porcine blood than bovine or ovine blood.
开展了多项研究，以确定用于体外饲养致痒血蜱成虫的血液的最佳更换间隔、储存时间和脊椎动物来源。在为期10天的实验期内，通过每只雌蜱产生的幼虫总数来衡量取食成功率，该实验期综合了成年雌蜱的存活、产卵和卵孵化情况。当血液更换间隔从24小时增加到48小时时，每只雌蜱产生的幼虫数量显著减少；然而，如果血液持续搅拌，血液更换间隔可增加到48小时而不影响幼虫产量。当成年苍蝇取食保存在3摄氏度下2周的全血、枸橼酸化牛血时，幼虫产量最高；然而，当成年苍蝇取食保存在-20摄氏度下2至52周的血液时，每只雌蜱产生的幼虫数量没有差异。当成年苍蝇取食枸橼酸化猪血时，产生的幼虫明显多于取食牛血或羊血时。
