体外在青霉素存在的情况下检测肺炎球菌荚膜抗原。

Detection of pneumococcal capsular antigen in the presence of penicillin in vitro.

作者信息

Holloway Y, Boersma W G, Kuttschrütter H, Snijder J A

机构信息

Department of Immunology Research, Regional Public Health Laboratory, Groningen, The Netherlands.

出版信息

Scand J Infect Dis. 1993;25(3):317-22. doi: 10.3109/00365549309008505.

DOI:10.3109/00365549309008505

PMID:8362227

Abstract

Eight strains of Streptococcus pneumoniae were tested in vitro for their ability to produce capsular antigen in the presence of penicillin. It was found that, provided 10(6) to 10(7) pneumococci/ml were present, capsular antigen could be detected during the 72 h in which the experiment was conducted, irrespective of whether penicillin was added at 0 h or 8 h, and even when no viable pneumococci remained. When fewer pneumococci were present, capsular antigen could not be detected at any time in the presence of penicillin. Control cultures, without penicillin, yielded detectable capsular antigen only when the threshold value of 10(6)-10(7) pneumococci/ml was reached. It is concluded that the presence of penicillin does not influence the detection of pneumococcal capsular antigen, but demonstration of this antigen is totally dependent on the number of pneumococci present.

摘要

对8株肺炎链球菌进行了体外测试，以检测它们在青霉素存在的情况下产生荚膜抗原的能力。结果发现，只要每毫升存在10⁶至10⁷个肺炎球菌，在进行实验的72小时内就能检测到荚膜抗原，无论青霉素是在0小时还是8小时添加，甚至当没有存活的肺炎球菌残留时也是如此。当肺炎球菌数量较少时，在青霉素存在的情况下任何时候都检测不到荚膜抗原。没有青霉素的对照培养物，只有当达到每毫升10⁶ - 10⁷个肺炎球菌的阈值时才会产生可检测到的荚膜抗原。得出的结论是，青霉素的存在并不影响肺炎球菌荚膜抗原的检测，但这种抗原的显示完全取决于存在的肺炎球菌数量。